Method For Treating Wounds With Enriched Platelet Wound Healant
An improved method for treating wounds with an enriched platelet composition is provided in which a small amount of a patient's blood is used to prepare, at point-of-care, a platelet-rich plasma (PRP) from which a gel is produced. The method involves the use of improved materials to improve the ease and speed at which wounds may be treated at the point-of-care.
1 . A method of treating a wound of a patient comprising:
withdrawing a predetermined amount of whole blood from the patient;
separating the whole blood into red blood cells, white blood cells and platelet-rich plasma;
extracting the separated platelet-rich plasma;
contacting an effective amount of ascorbic acid to the platelet-rich plasma;
contacting an effective amount of calcified thrombin to the extracted platelet-rich plasma to activate the platelets and to obtain a gel; and
contacting an effective amount of the gel to the wound.
2 . The method of claim 1 wherein the step of withdrawing a predetermined amount of whole blood from the patient comprises withdrawing the predetermined amount of whole blood from the patient into one or more blood collection tubes configured to be spun in a centrifuge at a point-of-care.
3 . The method of claim 2 wherein the step of separating the whole blood into red blood cells, white blood cells, and platelet-rich plasma comprises spinning the one or more blood collection tubes in a centrifuge configured to be operated at the point-of-care.
4 . The method of claim 3 wherein the step of spinning the one or more blood collection tubes in a centrifuge configured to be operated at the point-of-care comprises spinning the one or more blood collection tubes in the centrifuge for about 1.5 minutes at the point-of-care.
5 . The method of claim 4 wherein the step of extracting the separated platelet-rich plasma comprises extracting the separated platelet-rich plasma into a syringe.
6 . The method of claim 5 wherein the step of contacting an effective amount of ascorbic acid to the platelet-rich plasma comprises adding the ascorbic acid into the syringe and inverting the syringe to mix.
7 . The method of claim 6 wherein the step of contacting an effective amount of calcified thrombin to the extracted platelet-rich plasma to activate the platelets and to obtain a gel comprises adding the calcified thrombin into the syringe and inverting the syringe several times to mix.
8 . The method of claim 1 wherein the step of withdrawing a predetermined amount of whole blood from the patient comprises withdrawing about 5 to about 30 cc of whole blood from the patient.
9 . The method of claim 8 wherein the step of withdrawing a predetermined amount of whole blood from the patient comprises withdrawing about 20 cc of whole blood from the patient.
10 . The method of claim 8 wherein the step of extracting the separated platelet-rich plasma comprises extracting about 8 mL of the separated platelet rich plasma.
11 . The method of claim 10 wherein the step of contacting an effective amount of ascorbic acid to the platelet-rich plasma comprises contacting about 1 mL of ascorbic acid to the platelet-rich plasma.
12 . The method of claim 11 wherein the step of contacting an effective amount of calcified thrombin to the extracted platelet-rich plasma to activate the platelets and to obtain a gel comprises contacting about 1 mL of calcified thrombin to the extracted platelet-rich plasma.
13 . A system for making a composition for treating a wound of a patient at a point-of-care comprising:
a blood-drawing device for drawing a predetermined amount of whole blood from the patient into one or more blood collection tubes;
one or more blood collection tubes configured to be spun in a centrifuge;
a centrifuge configured to operate at the point-of-care to separate the whole blood into red blood cells, white blood cells and platelet-rich plasma;
an extracting device for extracting a predetermined amount of the separated platelet-rich plasma from the one or more blood collection tubes; and
a mixing device for coupling to the extracting device and adding a predetermined amount of one or more reagents to the separated platelet rich plasma.