Target vector with activable imaging function
The invention concerns the field of molecular probe architecture for in vivo imaging. More particularly, the invention concerns molecular constructs providing an imaging function activable in intracellular environment. The inventive fluorescence probes enable in particular images of certain targeted tissues to be formed, while maintaining a low background noise level and, preferably, while obtaining at the targeted tissue, an imaging signal increasing in time.
1. A targeted biological vector comprising:
a Regioselectively Addressable Functionalized Template (RAFT) cyclodecapeptide vector core bound to:
(i) at least one targeting molecule which is a peptide that comprises a RGD motif, and
(ii) at least one activatable imaging function provided by a first fluorophore linked to a fluorescence quencher by an arm that is cleavable in an intracellular medium;
wherein said arm comprises two adjacent cysteine residues bound by a disulfide bridge; and
wherein said fluorophore is Cyanine 5 (Cy5), and said fluorescent quencher is QSY21.
2. The vector of claim 1 , wherein said targeting molecule recognizes a receptor overexpressed on a surface of a cell.
3. The targeted biological vector of claim 1 , wherein the targeting molecule is a ligand for a molecule over-expressed by a tumor cell or endothelial cell during neoangiogenesis.
4. The vector of claim 1 , wherein said at least one targeting molecule is a cRGD cyclopeptide.
5. The vector of claim 1 , wherein said at least one targeting molecule is an RGD peptide selected from the group consisting of cyclo(RGDfK), cyclo(RGDyK) and cyclo(RDGfV).
6. The vector of claim 1 , wherein said at least one targeting molecule comprises an RGD peptide that recognizes an α v β 3 integrin that is expressed on the surface of a tumor cell.
7. The vector of claim 1 , wherein said at least one targeting molecule comprises an RGD peptide that recognizes an α v β 3 integrin that is expressed on the surface of an endothelial cell during tumor neoangiogenesis.
8. The vector of claim 1 , wherein said at least one targeting molecule comprises an RGD peptide selected from the group consisting of octeotrate peptide, a peptide analog of somatostatin, a peptide analog of bombesin, EGF (epidermal growth factor) or VIP (vasoactive intestinal peptide).
9. A vector comprising:
a Regioselectively Addressable Functionalized Template (RAFT) cyclodecapeptide molecular vector bound to:
(i) at least one targeting molecule which is a peptide that comprises a RGD motif, and
(ii) an activatable imaging function comprising a fluorophore, a fluorescence quencher and an arm;
wherein the fluorophore is Cyanine 5 (Cy5), and the fluorescence quencher is QSY21;
wherein the fluorophore and fluorescence quencher are linked by said arm; and
wherein said arm comprises two adjacent cysteine residues bound by a disulfide bridge and is cleavable in an intracellular medium.
10. The vector of claim 9 , wherein said at least one targeting molecule is a cRGD cyclopeptide.
11. The vector of claim 9 , wherein said at least one targeting molecule is an RGD peptide selected from the group consisting of cyclo(RGDfK), cyclo(RGDyK) and cyclo(RDGfV).
12. The vector of claim 9 , wherein said at least one targeting molecule comprises an RGD peptide that recognizes an α v β 3 integrin that is expressed on the surface of a tumor cell.
13. The vector of claim 9 , wherein said at least one targeting molecule comprises an RGD peptide that recognizes an α v β 3 integrin that is expressed on the surface of an endothelial cell during tumor neoangiogenesis.
14. The vector of claim 9 , wherein said at least one targeting molecule comprises an RGD peptide selected from the group consisting of octeotrate peptide, a peptide analog of somatostatin, a peptide analog of bombesin, EGF (epidermal growth factor) or VIP (vasoactive intestinal peptide).