Compositions of and methods of using sulfatases from flavobacterium heparinum
This invention is related, in part, to sulfatase enzymes and methods of their use.
1. An isolated nucleic acid molecule which codes for 6-O-sulfatase or N-sulfamidase selected from the group consisting of:
(a) nucleic acid molecules which hybridize at 65° C. in hybridization buffer (3.5×SSC (0.15 M sodium chloride/0.015 M sodium citrate, pH 7), 0.02% Ficoll, 0.02% polyvinyl pyrrolidone, 0.02% Bovine Serum Albumin, 2.5 mM NaH 2 PO 4 (pH 7), 0.5% sodium dodecyl sulphate (SDS), 2 mM ethylenediaminetetracetic acid (EDTA)); and after washing first in 2×SSC at room temperature and second in 0.1-0.5×SSC/0.1×SDS at 68° C. to a nucleic acid molecule having a nucleotide sequence set forth in SEQ ID NO: 1 or 3;
(b) nucleic acid molecules that differ from the nucleic acid molecules of (a) in codon sequence due to degeneracy of the genetic code, and
(c) full complements of (a) or (b).
2. An isolated nucleic acid molecule having a nucleotide sequence set forth in SEQ ID NO: 1.
3. An isolated nucleic acid molecule having a nucleotide sequence set forth in SEQ ID NO: 3.
4. An expression vector comprising the isolated nucleic acid molecule of any of claims 1 - 3 operably linked to a promoter.
5. A host cell comprising the expression vector of claim 4 .