Diarylalkanes as potent inhibitors of binuclear enzymes
The present invention implements a strategy that combines an enzyme inhibition assay with a chemical dereplication process to identify active plant extracts and the particular compounds—diarylalkanes and/or diarylalkanols within those extracts that specifically inhibit binuclear enzyme function. Included in the present invention are compositions of matter comprised of one or more of diarylalkanes and/or diarylalkanols, which inhibit the activity of binuclear enzymes, particularly tyrosinase and which prevent melanin overproduction. The present invention also provides a method for inhibiting the activity of a binuclear enzyme, particularly tyrosinase and a method for preventing and treating diseases and conditions related to binuclear enzyme function. The present invention further includes a method for preventing and treating melanin overproduction and diseases and conditions of the skin related thereto. The method for preventing and treating diseases and conditions related to binuclear enzyme function and melanin overproduction is comprised of administering to a host in need thereof an effective amount of a composition comprising one or more diarylalkanes and/or diarylalkanols synthesized and/or isolated from one or more plants together with a pharmaceutically acceptable carrier.
1. A method for inhibiting the activity of tyrosinase said method comprising administering to a subject in need thereof an effective amount of a composition comprising 1-(3-methyl-2,4-dimethoxyphenyl)-3-(2′,4′-dihydroxyphenyl)-propane.
2. The method of claim 1 wherein said 1-(3-methyl-2,4-dimethoxyphenyl)-3-(2′,4′-dihydroxyphenyl)-propane is obtained by organic synthesis or is isolated from one or more plants.
3. The method of claim 2 wherein said 1-(3-methyl-2,4-dimethoxyphenyl)-3-(2′,4′-dihydroxyphenyl)-propane is isolated from a plant part selected from the group consisting of stems, stem barks, trunks, trunk barks, twigs, tubers, roots, root barks, young shoots, seeds, rhizomes, flowers and other reproductive organs, leaves and other aerial parts.
4. The method of claim 2 wherein said 1-(3-methyl-2,4-dimethoxyphenyl)-3-(2′,4′-dihydroxyphenyl)-propane is isolated from a plant family selected from the group consisting of Compositae, Fabaceae, Lauraceae, Leguminosae, Liliaceae, Loranthaceae, Moracea, and Myristicaceae.
5. The method of claim 2 wherein said 1-(3-methyl-2,4-dimethoxyphenyl)-3-(2′,4′-dihydroxyphenyl)-propane is isolated from a plant genus selected from the group consisting of Acacia, Broussonetia, Dianella, Helichrysum, Iryanthera, Knema, Lindera, Pterocarpus, Viscum , and Xanthocercis.
6. The method of claim 1 wherein said administration inhibits excessive pigmentation of the skin, lightens age spots, sun spots, acne spots or liver spots.
7. The method of claim 1 wherein said administration lightens dark skin pigmentation, reduces skin dullness, evens skin tone or corrects skin discoloration.
8. The method of claim 1 wherein said administration reduces signs of aging on the skin and reduces wrinkles associated with aging by neutralization of active oxygen species.
9. The method of claim 1 wherein said administration inhibits the synthesis of melanin.