Microorganisms Inhibiting the Formation of Axillary Malodor
Described are microorganisms which are able to suppress the production of malodorous compounds by axillary bacteria. Also described are compositions comprising such microorganisms as well as the use of such micoorganisms in cosmetic, prophylactic or therapeutic applications.
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16 . A microorganism that is capable of suppressing the release of 3-methyl-2-hexenoic acid or its odorous derivatives by axillary bacteria.
17 . The microorganism of claim 16 , wherein the microorganism shows the capability of suppressing the release of 3-methyl-2-hexenoic acid or its odorous derivatives by axillary bacteria by at least one of the following assays:
Assay (A):
(i) mixing the microorganism with a microorganism that is capable of releasing 3-methyl-2-hexenoic acid or an odorous derivative thereof and with an odorless axillary secret;
(ii) incubating the mixture under conditions allowing the release of 3-methyl-2-hexenoic acid;
(iv) extracting short fatty acids from the supernatant of the mixture; and
(iv) detecting odor release by the occurrence of 3-methyl-2-hexenoic acid;
or
Assay (B):
(i) mixing said microorganism with a microorganism which is capable of releasing 3-methyl-2-hexenoic acid or an odorous derivative thereof and with N-α-lauryl-glutamine;
(ii) incubating the mixture under conditions allowing the release of laureate derived from N-α-lauryl-glutamine; and
(iv) analyzing the supernatant of the mixture for the presence of N-α-lauryl-glutamine, or
Assay (C):
a sniffing assay with the nose.
18 . The microorganism of claim 16 , wherein the 3-methyl-2-hexenoic acid or its odorous derivatives is released by a bacterium in the genus Corynebacterium.
19 . The microorganism of claim 18 , wherein the bacterium is Corynebacterium jeikeium.
20 . The microorganism of claim 16 , wherein the bacterium is in the genus Lactobacillus.
21 . The microorganism of claim 20 , wherein the bacterium is selected from the group consisting of Lactobacillus plantarum, Lactobacillus crispatus, Lactobacillus acidophilus II, Lactobacillus acidophilus III and Lactobacillus delbruckii delbruckii.
22 . The microorganism of claim 21 , wherein the bacterium is selected from the group consisting of Lactobacillus plantarum OB-AG-0002 (DSM 17598), Lactobacillus crispatus OB-AG-0003 (DSM 17567), Lactobacillus acidophilus II OB-AG-0004 (DSM 17568), Lactobacillus acidophilus II OB-AG-0005 (DSM 17569), Lactobacillus acidophilus III OB-AG-0006 (DSM 17570) and Lactobacillus delbrückii delibrückii OB-AG- 0007 (DSM 17571) or a mutant or derivative thereof, and wherein the mutant or derivative retains the ability to suppress the release of 3-methyl-2-hexenoic acid or its odorous derivatives by axillary bacteria.
23 . The microorganism of claim 16 , wherein the microorganism is an inactive form and able to suppress the release of 3-methyl-2-hexenoic acid or its odorous derivatives by axillary bacteria.
24 . The microorganism of claim 23 , wherein the microorganism further is thermally inactivated or lyophilized.
25 . A composition comprising a microorganism selected from the group consisting of the microorganism of claim 16 , the microorganism of claim 23 , and the microorganism of claim 24 .
26 . The composition of claim 25 , wherein the composition is a cosmetic composition, optionally comprising a cosmetically acceptable carrier or excipient.
27 . The composition of claim 25 , wherein the composition is a pharmaceutical composition, optionally comprising a pharmaceutically acceptable carrier or excipient.
28 . A method for the production of a cosmetic composition comprising the step of formulating a microorganism selected from the group consisting of the microorganism of claims 16 , 17 , 18 , 19 , 21 , 22 , 23 and 24 with a cosmetically acceptable carrier or excipient.
29 . A method for the production of a pharmaceutical composition comprising the step of formulating a microorganism selected from the group consisting of the microorganism of claims 16 , 17 , 18 , 19 , 21 , 22 , 23 and 24 with a pharmaceutically acceptable carrier or excipient.