IP Library › Granted Patent US 7,613,583
Granted Patent B2
US 7,613,583 · App. 12/071,580 · Granted Nov 3, 2009

Method and system for particle data processing

Assignee: Beckman Coulter, Inc.
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Quick Facts
Patent No.
US 7,613,583
App. No.
12/071,580
Granted
Nov 3, 2009
Kind
B2
Abstract

In an embodiment, a computer-implemented method is provided for processing digital data from a particle analyzer. The method includes receiving digital particle data, transforming the received data to correct for noise, applying linear compensation for dye spectral overlap, and re-establishing the original dimensions of the data. Processed particle data may be used for display, analysis or storage. In another embodiment, a system comprises at least one particle analyzer coupled to a particle data corrector. The particle data corrector includes a transformer, a compensator, and a converter.

Claims (34)

1. A computer-implemented method for processing digital data from a particle analyzer, comprising:

receiving raw particle data from the particle analyzer at a computer, wherein the raw particle data has noise including photon counting statistics error;

transforming the raw particle data to obtain transformed particle data having reduced photon counting statistics error;

compensating the transformed particle data for dye spectral overlap, to obtain compensated particle data;

converting the compensated particle data to obtain converted particle data, having dimensions conforming to the raw particle data; and

outputting the converted particle data.

2. The method of claim 1 , further comprising displaying the converted particle data.

3. The method of claim 1 , further comprising storing the converted particle data.

4. The method of claim 1 , wherein the transforming comprises using at least one mathematical function to obtain the transformed particle data.

5. The method of claim 4 , wherein the converting comprises using at least one function that is the inverse of said mathematical function.

6. The method of claim 1 , wherein compensating the transformed particle data for dye spectral overlap comprises using at least one linear mathematical function.

7. The method of claim 2 , wherein the displaying includes displaying the converted data using a logarithmic scale or biexponential scale.

8. A system for processing particle data from at least one light detector, comprising:

at least one particle analyzer;

a particle data corrector that receives from the at least one particle analyzer raw particle data having noise including photon counting statistics error;

a display device, wherein the display device is coupled with the particle data corrector; and

wherein the particle data corrector includes:

a transformer that transforms the raw particle data to generate transformed particle data having reduced photon counting statistics error;

a compensator that compensates the transformed particle data to generate compensated particle data having reduced dye spectral overlap; and

a converter that converts the compensated particle data to generate converted particle data having dimensions conforming to said raw particle data.

9. The system of claim 8 , wherein the particle analyzer comprises a flow cytometer.

10. The system of claim 8 , wherein the transformer applies at least one mathematical function to obtain the transformed particle data.

11. The system of claim 10 , wherein the converter applies at least one function that is the inverse of the said mathematical function.

12. The system of claim 8 , wherein the compensator applies at least one linear mathematical function.

13. A system for reducing noise in raw particle data generated from at least one light detector, the noise including photon counting statistics error, said system comprising:

a transformer that transforms the raw particle data to generate transformed particle data having reduced photon counting statistics error;

a compensator that compensates the transformed particle data to generate compensated particle data having reduced dye spectral spillover effects;

a converter that processes the compensated particle data to generate converted particle data having dimensions conforming to said raw particle data; and

an output interface that outputs the converted particle data.

14. A computer program product comprising a computer usable medium having control logic stored therein for causing a computer to process raw particle data from at least one light detector, the raw particle data having noise including photon counting statistics error, said control logic comprising:

first computer readable program code that enables the computer to transform the raw particle data to generate transformed particle data having reduced photon counting statistics error;

second computer readable program code that enables the computer to compensate the dye spectral overlap of the corrected particle data to generate compensated particle data;

third computer readable program code that enables the computer to convert the compensated particle data to generate converted particle data having dimensions of the raw particle data; and

fourth computer readable program code to enable the computer to output the converted particle data.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 22, 2008
From: ESTEVEZ-LABORI, FRANCISCO
To: BECKMAN COULTER, INC.
Reel/Frame 020603/0295 →
Continuity (1)
Related Publication 20090216478A1 · Aug 27, 2009