Electrophoresis device and electrophoresis method
View Patent ↗To reduce the elongation of the migration time for the first electrophoresis cycle in successive electrophoresis cycles and improve the reliability of electrophoresis analysis. The present invention relates to making the condition of a migration medium at the start of the first electrophoresis analysis in successive electrophoresis analyses after the temperature in a thermostatic oven reaches a desired preset temperature substantially the same as the conditions after the successive electrophoresis analyses. Preferably, a voltage is applied to a separation medium filling a capillary during preheating of the thermostatic oven. Preferably, the temperature in the thermostatic oven during preheating is set higher than the temperature in the thermostatic oven during electrophoresis analysis. Preferably, a buffer solution is heated during preheating of the thermostatic oven. Preferably, the capillary is filled with a preheated separation medium during preheating of the thermostatic oven. According to the present invention, the reliability of the first electrophoresis analysis in successive electrophoresis analyses is improved.
1. An electrophoresis device, comprising:
a capillary capable of being filled with a separation medium;
a thermostatic oven that houses at least a part of the capillary and adjusts the temperature of the capillary; and
a power supply mechanism capable of applying a voltage at least between the opposite ends of the capillary,
wherein said power supply mechanism applies a voltage to the separation medium filling said capillary during preheating of the thermostatic oven.
2. The electrophoresis device according to claim 1 , wherein the temperature of the thermostatic oven during preheating is higher than the temperature of the thermostatic oven during electrophoresis analysis.
3. The electrophoresis device according to claim 1 , further comprising:
a separation medium filling mechanism capable of filling the capillary with the separation medium,
wherein the capillary is filled with a preheated separation medium during preheating of the thermostatic oven.
4. The electrophoresis device according to claim 1 ,
wherein the temperature of the thermostatic oven during preheating is higher than the temperature of the thermostatic oven during electrophoresis analysis.
5. An electrophoresis method, comprising:
a step of preparing a capillary that is filled with a separation medium and at least a part of which is housed in a thermostatic oven;
a step of applying a voltage to the separation medium filling the capillary during preheating of the thermostatic oven; and
a step of performing an electrophoresis analysis after the temperature in the thermostatic oven reaches a temperature suitable for the electrophoresis analysis.