Acoustic assessment of characteristics of a fluid relevant to acoustic ejection
Methods are provided for analyzing characteristics of fluids in the context of an acoustic ejection system. Such a system has a controller, an acoustic radiation generator, and a coupling medium coupling the radiation to a reservoir holding fluid. The methods can use acoustic radiation to both perturb a surface of the fluid in the reservoir and analyze the effect of the perturbation. The methods may use information about prior fluids. The methods of the invention can determine physical characteristics such as speed of sound and viscosity. The methods also include ways to determine a level of acoustic energy suitable to eject a droplet. Preferably the methods are executed automatically under control of programming of a controller of an acoustic ejection system.
1. A method of determining an energy level suitable for ejecting a droplet of a fluid with a particular toneburst waveform, comprising the steps of:
(a) sending focused acoustic radiation with said toneburst waveform to a place near a surface of a quantity of the fluid in order to eject said droplet,
(b) subsequent to step (a) sending a plurality of probe pulses towards the surface of the quantity of the fluid,
(c) detecting a plurality of echoes from the plurality of probe pulses, and
(d) based on at least an analysis of said plurality of echoes detected in (c), determining whether the energy level related to said toneburst waveform was sufficient to eject said droplet.
2. The method of claim 1 , where step (d) comprises the steps of:
(d1) distinguishing the plurality of echoes from the plurality of probe pulses into at least a primary echo and a further echo, and
(d2) using the temporal duration of the further echo as an input to determine whether ejection took place.
3. The method of claim 1 , where step (d) comprises the steps of:
(d1) distinguishing the plurality of echoes from the plurality of probe pulses into at least a primary echo and a further echo, and
(d2) determining whether ejection took place using as an input whether the temporal difference between the detected primary echo and the further echo tended to diminish with increasing time following the execution of step (a).
4. The method of claim 1 where the quantity of the fluid used in step (a) is less than 50 μL.
5. The method of claim 1 where the quantity of the fluid used in step (a) is less than 10 μL.
6. The method of claim 1 where the total time taken to perform the method is no more than 1 minute from the time at which the acoustic radiation is first directed at the quantity of the fluid in the course of performing the method.
7. The method of claim 6 where the total time taken to perform the method is no more than 3.8 seconds from the time at which the acoustic radiation is first directed at the quantity of the fluid in the course of performing the method.
8. The method of claim 1 , where step (d) comprises the steps of:
(d1) performing a Fourier-type transform algorithm taking as an input an echo of a probe pulse detected in (c), and
(d2) computing an estimate of an ejection threshold, taking as an input an output of the Fourier-type transform algorithm performed in step (d1).
9. The method of claim 8 , where step (d2) comprises the step of identifying a minima of an output of the Fourier-type transformation performed in step (d 1).
10. A method of determining an energy level suitable for ejecting a droplet of a fluid with a particular toneburst waveform, comprising the steps of:
(a) sending focused acoustic radiation with said toneburst waveform to a place near a surface of a quantity of the fluid in order to eject said droplet,
(b) subsequent to step (a) sending a probe pulse towards the surface of the quantity of the fluid,
(c) detecting an echo from the probe pulse, and
(d) based on at least an analysis of the echo detected in (c), determining whether the energy level related to said toneburst waveform was sufficient to eject said droplet.
11. The method of claim 10 , where step (d) comprises the steps of:
(d1) performing a Fourier-type transform algorithm taking as an input the echo detected in (c), and
(d2) computing an estimate of an energy difference between the particular toneburst waveform and an ejection threshold, taking as an input an output of the Fourier-type transform algorithm performed in step (d 1).
12. The method of claim 11 , where step (d2) comprises the step of identifying a minima of an output of the Fourier-type transform algorithm performed in step (d1).
13. The method if claim 10 where the quantity of the fluid used in step (a) is less than 50 μL.