Forming or patterning composite microstructures using microfluidics
View Patent ↗Techniques comprising systems and methods for forming or pattering microstructures using microfluidics are described. A target structure can include a microchannel passage with a chamber into which a light hardenable material can be injected and selectively exposed to light to adhere to a desired location of a substrate. Unhardened material can be washed out of the chamber. One or more subsequent cycles can repeat the process using the same or a different material. A variety of composite microstructures can be created, including structures encapsulating viable living cells, such as for cell study or tissue engineering.
1. A method for forming a composite structure, the method comprising:
flowing a first precursor solution into an assembly region;
exposing at least a first portion of the assembly region to light such that the first precursor solution in at least said first portion of the assembly region is polymerized into a first polymer section;
flowing a second precursor solution into the assembly region; and
exposing at least a second portion of the assembly region to light such that the second precursor solution in at least said second portion of the assembly region is polymerized into a second polymer section,
wherein the first polymer section has a material property different from that of the second polymer section, and
the exposing at least a first portion to light and the exposing at least a second portion to light are performed using light from a confocal laser scanning microscope.
2. The method of claim 1 , wherein the material property is a porosity of the respective polymer section.
3. The method of claim 1 , wherein the material property is a stiffness of the respective polymer section.
4. The method of claim 1 , wherein the first polymer section has a different material composition from that of the second polymer section.
5. The method of claim 1 , wherein the first and second polymer sections form a composite, anisotropic hydrogel structure.
6. The method of claim 1 , wherein said assembly region is a region of a microfluidic channel having a dimension less than 1 mm.
7. The method of claim 1 , wherein the first and second precursor solutions include respective prepolymers and photoinitiators.
8. The method of claim 1 , wherein the first precursor solution or the second precursor solution includes a polyethylene glycol (PEG) based prepolymer.
9. The method of claim 1 , wherein the first precursor solution or the second precursor solution includes a polyethylene glycol based prepolymer with adhesion peptides.
10. The method of claim 1 , further comprising seeding at least one of the first and second polymer sections with living cells.
11. The method of claim 1 , wherein the assembly region has a height, and at least one of the first and second polymer sections has a height in a direction parallel to said assembly region height that is less than said assembly region height.
12. The method of claim 1 , wherein at least a portion of the first polymer section overlaps at least a portion of the second polymer section in plan view.