Bacterial growth enhancer
We describe the production and use of an extract obtained from Musa spp., preferably from bananas, in the promotion of growth of Gram-positive bacteria such as lactic acid bacteria. The extract is also useful for growth enhancement of environmentally-stressed Gram negative bacteria. Fermented foods containing such extracts are also described.
1. A method of enhancing or promoting growth or viability of bacteria selected from the group consisting of: Gram positive bacteria, and lactic acid bacteria, the method comprising:
preparing an extract obtained from Musa spp by:
blending at least a portion of a Musa fruit in a suitable diluent to form a liquidized intermediary;
centrifuging the liquidized intermediary to form a juice and a debris pellet;
decanting the juice from the debris pellet to form the extract; and
autoclaving the extract;
introducing an enhanced bacterial growth medium consisting of a serum-free bacterial growth medium supplemented with the extract to said bacteria, wherein the extract is present in said medium at a concentration of between 0.01 and 10%; and
culturing an isolated bacterial sample of said bacteria on said growth medium to thereby enhance or promote growth or viability of said bacteria over that which would be obtained with a medium typically used to grow said bacteria in the absence of said extract;
wherein said bacterial growth medium is capable of enhancing growth of said bacteria from an OD600 value of 0.1 to at least 0.145 over an incubation period of at least 1480 minutes, or
wherein said bacterial growth medium is capable of enhancing viability of said bacteria from 10 5 colony forming units (CFU)/ml to at least 10 7 CFU/ml over 7 days.
2. The method of claim 1 wherein the extract is obtained from banana skin and/or banana pulp.
3. The method of claim 1 wherein the bacteria are lactic acid bacteria.
4. The method of claim 1 wherein the bacteria are environmentally stressed prior to growth with the extract.
5. The method of claim 1 , wherein the culturing of the bacteria is in a non-anaerobic environment.
6. The method of claim 1 , wherein the sample is taken from a food product, or a brewing or winemaking product.
7. The method of claim 1 , wherein preparing the extract comprises any or all of the following additional steps: filtering the extract; drying or lyophilizing the extract; and freezing the extract.
8. A method, comprising:
introducing a bacterial growth medium selected from the group consisting of deMann Rogan Sharpe (MRS) and Luria, the bacterial growth medium supplemented with an extract obtained from a plant selected from the group consisting of: Musa spp, and a species of apple, orange, plum, carrot, tea, or coffee to bacteria, wherein the extract is present in said medium at a concentration of 0.01 to 10%; and
culturing an isolated bacterial sample of said bacteria on said growth medium to thereby enhance adherence to a substrate, or to promote viability, or viscosity, said bacteria being selected from the group consisting of: Gram positive bacteria and lactic acid bacteria,
wherein said bacterial growth medium is capable of enhancing viability of said bacteria from 10 5 colony forming units (CFU)/ml to at least 10 7 CFU/ml over 7 days.
9. The method of claim 8 , wherein the extract is obtainable or obtained from Musa spp.
10. The method of claim 8 , wherein the bacteria are lactic acid bacteria.
11. The method of claim 8 , wherein the extract is present in said medium at a concentration of between about 0.1 to 5%.
12. The method of claim 11 , wherein the extract is present in said medium at a concentration of between about 1 to 2%.
13. The method of claim 8 , wherein the medium does not comprise serum, and wherein the supplement comprises no additional nutrient components.
14. The method of claim 8 , wherein the extract does not substantially comprise catecholamines and/or fructo-oligosaccharides.
15. The method of claim 8 , further comprising preparing the extract by:
blending at least a portion of a Musa fruit in a suitable diluent to form a liquidized intermediary;
centrifuging the liquidized intermediary to form a juice and a debris pellet;
decanting the juice from the debris pellet to form the extract; and
autoclaving the extract.
16. The method of claim 1 , wherein the extract is present in said medium at a concentration of between about 1 to 2%.
17. The enhanced bacterial growth medium of claim 1 , wherein the bacterial growth medium is selected from the group consisting of MRS and Luria.
18. The method of claim 1 , wherein the extract is autoclaved at 121° C. and 103 kPa for 15 minutes.