Increasing the viability of mammalian oocytes
The present invention relates to a method for improving viability and/or stress tolerance of viable biological material and using the said material comprising applying hydrostatic pressure to said biological material; keeping the said viable biological material at the hydrostatic pressure for a predetermined time period; releasing the hydrostatic pressure; and using the said material for any desired purpose in accordance with any useful protocol. The usage of the said biological material incorporates any techniques, protocols that are applicable in the field of assisted reproductive techniques, biotechnical and/or biotechnological manipulations.
1. A method for improving the viability of a viable biological material and using the material comprising:
(a) applying hydrostatic pressure to said biological material;
(b) keeping said viable biological material at the hydrostatic pressure for a predetermined time period;
(c) releasing the hydrostatic pressure;
(d) using the viable biological material in the field of assisted reproductive techniques,
with the proviso that no cryopreservation of the viable biological material is performed, and wherein the viable biological material is an oocyte of a mammal.
2. The method according to claim 1 wherein said hydrostatic pressure is in the range of 1 to 200 MPa.
3. The method according to claim 2 wherein said hydrostatic pressure is applied for a time period between 10 seconds and 150 minutes.
4. The method according to claim 1 wherein said hydrostatic pressure is applied for a time period between 0.001 seconds and 600 minutes.
5. The method according to claim 4 wherein said hydrostatic pressure is in the range of 10 to 100 MPa.
6. The method according to claim 1 wherein said pressure is released gradually over a time period between instantaneous release and 6 hours.
7. The method according to claim 1 wherein said pressure is applied, kept and released according to a predetermined pressure profile.
8. The method according to claim 1 wherein said mammal is selected from the group consisting of bovine, equine, caprine, ovine, swine, other livestocks, pets, and primates.
9. The method according to claim 1 wherein said hydrostatic pressure is in the range of 10 to 100 MPa.
10. The method according to claim 1 wherein said hydrostatic pressure is in the range of 20 to 75 MPa.
11. The method according to claim 1 wherein said hydrostatic pressure is in the range of 30 to 60 MPa.
12. The method according to claim 1 wherein said hydrostatic pressure is applied for a time period between 1 second and 300 minutes.
13. The method according to claim 1 wherein said hydrostatic pressure is applied for a time period between 10 seconds and 150 minutes.
14. The method according to claim 1 wherein said hydrostatic pressure is applied for a time period between 20 seconds and 90 minutes.
15. The method according to claim 1 wherein said hydrostatic pressure is applied for a time period between 30 seconds and 60 minutes.