Reagents and methods for smooth muscle therapies
The present invention provides novel polypeptides comprising heat shock protein 20 (HSP20)-derived polypeptides to treat or inhibit smooth muscle vasospasm, as well to treat and inhibit smooth muscle cell proliferation and migration.
1. An isolated polypeptide represented by the following structural formula:
X2-SEQ ID NO: 300-X6
X2 is absent or is a cell transduction domain;
X6 is absent or is a cell transduction domain;
At least one of X2 and X6 is a transduction domain; and
(SEQ ID NO: 300) is Trp Leu Arg Arg Ala Ser Ala Pro Leu Pro Gly Leu Lys; and
the serine in SEQ ID NO: 300 is phosphorylated.
2. A pharmaceutical composition comprising the isolated polypeptide of claim 1 and a pharmaceutically acceptable carrier.
3. An isolated polypeptide represented by the following structural formula:
X2-SEQ ID NO: 300
X2 is a cell transduction domain;
(SEQ ID NO: 300) is Trp Leu Arg Arg Ala Ser Ala Pro Leu Pro Gly Leu Lys; and
the serine in SEQ ID NO: 300 is phosphorylated.
4. A pharmaceutical composition comprising the isolated polypeptide of claim 3 and a pharmaceutically acceptable carrier.