Carbohydrase expression during degradation of whole plant material by
The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and/or depolymerize cellulose, hemicellulose and other carbohydrates. These systems have a number of applications.
1. An isolated Saccharophagus degradans prokaryotic cell-free composition comprising Saccharophagus degradans lysate, said lysate comprising Cel5F, wherein the composition is effective in the degradation of plant material and wherein the Saccharophagus degradans is grown to an OD 600 of at least 0.3 in the presence of the plant material prior to lysis.
2. The Saccharophagus degradans prokaryotic cell-free composition of claim 1 , wherein the lysate further comprises Cel5H.
3. The Saccharophagus degradans prokaryotic cell-free composition of claim 1 , wherein the lysate further comprises Cel5I and Cep94A.
4. The Saccharophagus degradans prokaryotic cell-free composition of claim 3 , wherein the lysate further comprises Cel6A, Bgl3C and Cel9B.
5. The Saccharophagus degradans prokaryotic cell-free composition of claim 4 , wherein the lysate further comprises Cel5A, Cel5B, Cel5C, Cel5D, Cel5E, Cel5G, Cel5J, Cel9A, Bgl1A, Bgl1B, Ced3A, Cep94B and Ced3B.
6. An isolated Saccharophagus degradans prokaryotic cell-free composition comprising Saccharophagus degradans lysate, said lysate comprising Cel5F, Cel9A, Bgl1A and Ced3B, wherein the composition is effective in the degradation of plant material, and
wherein the Saccharophagus degradans is grown to an OD 600 of at least 0.3 in the presence of plant material prior to lysis.
7. The Saccharophagus degradans prokaryotic cell-free composition of claim 6 , wherein the lysate further comprises Cel5B, Cel5E, Cel5I, Bgl1B, Bgl3C, Cep94A and Cep94B.
8. An isolated Saccharophagus degradans prokaryotic cell-free composition comprising Saccharophagus degradans lysate, said lysate comprising Cel5E, Cel5F, Cel5H, Cel6A and Cel9B, wherein the composition is effective in the degradation of plant material, and
wherein the Saccharophagus degradans is grown to an OD 600 of at least 0.3 in the presence of plant material prior to lysis.
9. The Saccharophagus degradans prokaryotic cell-free composition of claim 8 , wherein the lysate further comprises Cel5I, Bgl3C and Cep94A.
10. The Saccharophagus degradans prokaryotic cell-free composition of claim 8 , wherein the lysate further comprises Cel5C, Cel5D and Ced3A.
11. The Saccharophagus degradans prokaryotic cell-free composition of any one of claim 1 , 6 or 8 , wherein the Saccharophagus degradans is grown to a OD 600 of from 0.3 to 10 in the presence of the plant material prior to lysis.
12. The Saccharophagus degradans prokaryotic cell-free composition of any one of claim 1 , 6 or 8 , wherein the Saccharophagus degradans is grown to a OD 600 of at least 0.370 in the presence of the plant material prior to lysis.
13. The Saccharophagus degradans prokaryotic cell-free composition of claim 12 , wherein the Saccharophagus degradans is grown to a OD 600 of from 0.370 to 0.400 in the presence of the plant material prior to lysis.