Apparatus for measuring high density lipoproteins and method of separating high density lipoproteins
View Patent ↗The present invention provides a method of separating lipoproteins other than high density lipoproteins from a biological fluid. The method can quickly measure HDL cholesterol with a simple configuration and without the need of providing additional complicated devices. In this method, high density lipoproteins not generating any precipitate are fractionated from low density lipoproteins, very-low density lipoproteins, and chylomicrons generating precipitates. Then the precipitates are removed not by centrifugal separation based on the conventional technology, but by filtration using a filter to separate high density lipoproteins in blood serum. A hydrophilic cellulose-mixed ester is preferable as a material for the filter, and the pore diameter is 0.8 μm or below. When the filtering method is employed, it is possible to eliminate the complicated operations required in the conventional centrifugal separation, and to shorten the time it takes for separation of the high density lipoproteins.
1. A method of separating lipoproteins other than high density lipoprotein (HDL) from a biological fluid, the method comprising the steps of:
mixing a biological fluid containing the lipoproteins other than HDL with a precipitant for the lipoproteins other than HDL to precipitate the lipoproteins other than HDL; and
circulating a precipitation liquid precipitated in the precipitating step through a carrier made of a mixture of nitrocellulose and cellulose acetate and having a pore diameter of 0.8 μm or below, wherein the precipitant is a polyanion in combination with a bivalent metal ion.
2. The method of separating lipoproteins other than HDL from a biological fluid according to claim 1 , wherein the step of mixing a biological fluid containing the lipoproteins other than HDL with a precipitant includes mixing the biological fluid with one of heparin-Ca precipitant, phosphotungustic acid-Mg precipitant, and dextran sulfate Mg-phosphotungustic acid-Mg precipitant to precipitate the lipoproteins other than HDL.