Microarray system
A microarray system is disclosed. The microarray system includes a microarray formed on a planar substrate and an incubation chamber formed around the microarray. The incubation chamber has a plurality of interior surfaces including a bottom surface on which the microarray is formed and a top surface that faces the bottom surface and is generally parallel to the bottom surface. At least one of a plurality of interior surfaces is a hydrophilic surface.
1. A microarray system, comprising:
a microarray formed on a planar substrate;
an incubation chamber comprising an enclosed space formed around said microarray, wherein said incubation chamber is connected to one inlet and one outlet channel;
a one-way valve for loading a liquid sample into said incubation chamber, wherein said one-way valve is connected to said incubation chamber through said inlet channel; and
a waste chamber in fluid communication with said incubation chamber through said outlet channel,
wherein said incubation chamber comprises a plurality of interior surfaces including a bottom surface on which said microarray is formed and a top surface that faces said microarray,
wherein said top surface is a hydrophilic surface and said bottom surface is a hydrophilic surface and wherein said top and bottom hydrophilic surfaces overcome the surface tension of a water-based liquid and allows complete filling of said incubation chamber with said water-based liquid,
wherein said outlet channel comprises an inlet section, a funnel shape connecting section, and an outlet section, wherein said inlet section has a diameter that is larger than a diameter of said outlet section, and
wherein said waste chamber has a volume that is larger than a volume of said incubation chamber and comprises an absorbent capable of wicking liquid from said incubation chamber,
wherein said incubation chamber is formed by placing a gasket around said microarray and covering said gasket with a hydrophilic tape or a hydrophilic film, wherein said top surface consists of the hydrophilic tape or the hydrophilic film.
2. The microarray system of claim 1 , wherein said hydrophilic tape or hydrophilic film is transparent.
3. The microarray system of claim 1 , wherein said bottom surface comprises impregnated chemicals that lyses cell membranes.
4. The microarray system of claim 3 , wherein said bottom surface comprises a hydrophilic matrix that retains nucleic acid from lysed cells.
5. The microarray system of claim 1 , wherein said one-way valve is a check valve.
6. The microarray system of claim 1 , wherein said one-way valve is a dome valve.
7. The microarray system of claim 1 , wherein said absorbent comprises cellulose.
8. The microarray system of claim 1 , wherein said absorbent is placed at a distance from said outlet channel to control wicking rate.
9. The microarray system of claim 1 , wherein said waste chamber is vented to atmosphere through a venting channel.
10. The microarray system of claim 1 , wherein said substrate is glass.
11. The microarray system of claim 1 , wherein said substrate is plastic.
12. The microarray system of claim 1 , wherein said microarray is an oligonucleotide array.
13. The microarray system of claim 1 , wherein said microarray is a protein array.
14. The microarray system of claim 13 , wherein said protein array is an antibody array.
15. The microarray system of claim 1 , wherein said microarray is formed by a gel spot printing method.