Methods for producing microRNAs
The invention relates to recombinant vectors for inducible and/or tissue specific expression of double-stranded RNA molecules that interfere with the expression of a target gene. In certain embodiments, the invention relates to the use of Tet (tetracycline)-responsive RNA Polymerase II (Pol II) promoters (e.g., TetON or TetOFF) to direct inducible knockdown in certain cells of an integrated or an endogenous gene, such as p53. The invention also relates to a method for producing transgenic animals (e.g., mice) expressing inducible (such as tetracycline-regulated), reversible, and/or tissue-specific double-stranded RNA molecules that interfere with the expression of a target gene.
1. A method for inhibiting expression of a target gene in a cell in an animal comprising:
(a) delivering a nucleic acid construct to an isolated cell so as to obtain a transformed cell harboring only a single, stably integrated copy of the construct, wherein said nucleic acid construct contains only a single tetracycline responsive RNA Polymerase II (Pol II) promoter operably linked to a sequence encoding only a single synthetic miR30-based shRNA directed against a target gene,
(b) culturing the transformed cell so as to obtain a population of transformed cells harboring only a single, stably integrated copy of the construct,
(c) implanting the transformed cells into an animal, and
(d) inducing expression of the miR30-based shRNAso as to inhibit expression of the target gene by at least about 80% in the implanted, transformed cells.
2. The method of claim 1 , wherein the promoter comprises a tet-off promoter and the miR30-based shRNA is expressed in the absence of tetracycline or a tetracycline analog.
3. The method of claim 2 , wherein the tetracycline analog is doxycyline.
4. The method of claim 1 , wherein the promoter comprises a tet-on promoter and the miR30-based shRNA is expressed by treating the recipient animal with tetracycline or a tetracycline analog.
5. The method of claim 4 , wherein the tetracycline analog is doxycycline.
6. The method of claim 2 , wherein inhibition of target gene expression is reversible.
7. The method of claim 4 , wherein inhibition of target gene expression is reversible.
8. The method of claim 1 , wherein the step of delivering the nucleic acid construct to the isolated cell is performed by liposome fusion.
9. The method of claim 1 , wherein the step of delivering the nucleic acid construct to the isolated cell is performed by transfection.
10. The method of claim 1 , wherein the step of delivering the nucleic acid construct to the isolated cell is performed by infection with a viral vector.
11. The method of claim 10 , wherein the viral vector is delivered at a multiplicity of infection yielding an infection efficiency of about 10% or less.