Multidimensional bioseparation with modular microfluidics
A multidimensional chemical separation and analysis system is described including a prototyping platform and modular microfluidic components capable of rapid and convenient assembly, alteration and disassembly of numerous candidate separation systems. Partial or total computer control of the separation system is possible. Single or multiple alternative processing trains can be tested, optimized and/or run in parallel. Examples related to the separation and analysis of human bodily fluids are given.
1. A system for performing multidimensional sequential chemical separations comprising:
at least one Rapid Prototyping Platform wherein one or more of said at least one Rapid Prototyping Platform includes a plurality of holes therein having a standard geometry and size suitable for receiving fasteners for attaching components to said one or more of said at least one Rapid Prototyping Platform and at least one adjustable spacer configured to elevate one or more of the components above a surface of said one or more of said at least one Rapid Prototyping Platform;
a plurality of interconnected Modular Microfluidic Components attached to said one or more of said at least one Rapid Prototyping Platform by means of fasteners suited for engaging one or more of said plurality of holes wherein said Modular Microfluidic Components are interconnected so as to form a chemical separation system capable of receiving a sample through an input port and capable of delivering one or more fractions to one or more output ports of said separation system, and wherein said plurality of interconnected Modular Microfluidic Components includes three or more Modular Microfluidic Components connected sequentially, and wherein said fasteners engaging said one or more of said plurality of holes in said one or more of said at least one Rapid Prototyping Platform are capable of rapid attachment and detachment from said holes thereby permitting effective system disassembly and reassembly into a different prototype system, optionally including one or more different Modular Microfluidic Components; and
a plurality of computer controlled valves, wherein each of the plurality of computer controlled valves are in fluid communication with one of the three or more Modular Microfluidic Components and one of a plurality of waste systems, and wherein each of the plurality of computer controlled valves are capable of automatically controlling, in a first setting, a flow of the sample or one or more fractions between each of the three or more Modular Microfluidic Components, and in a second setting, unwanted materials to one of the plurality of waste systems.
2. A system as in claim 1 further comprising at least one device for performing analysis interconnected to at least one of said one or more output ports.
3. A system as in claim 1 further comprising a computer connected to one or more of said Modular Microfluidic Components for automatic control of said one or more Modular Microfluidic Components.
4. A system as in claim 1 wherein said device for performing analysis is an on-chip analytical device capable of being attached to said at least one Rapid Prototyping Platform by means of fasteners suited for engaging one or more of said plurality of holes.
5. A method of performing multidimensional sequential chemical separations comprising:
providing at least one Rapid Prototyping Platform wherein said Rapid Prototyping Platform includes a plurality of holes therein having a standard geometry and size suitable for receiving fasteners for attaching components to said Rapid Prototyping Platform and at least one adjustable spacer configured to elevate one or more the components above a surface of said one or more of said at least one Rapid Prototyping Platform;
providing a plurality of interconnected Modular Microfluidic Components attached to said at least one Rapid Prototyping Platform wherein said Modular Microfluidic Components are interconnected so as to form a chemical separation system, and wherein said plurality of interconnected Modular Microfluidic Components includes three or more Modular Microfluidic components connected sequentially, and wherein said fasteners engaging said one or more of said plurality of holes in said Rapid Prototyping Platform are capable of rapid attachment and detachment from said holes thereby permitting effective system disassembly and reassembly into a different prototype system, optionally including one or more different Modular Microfluidic Components;
delivering a sample through at least one input port into a first component of said plurality of interconnected Modular Microfluidic Components;
receiving one or more fractions at one or more output ports of said chemical separation system; and
a plurality of computer controlled valves, wherein each of the plurality of computer controlled valves are in fluid communication with one of the three or more Modular Microfluidic Components and one of a plurality of waste systems, and wherein each of the plurality of computer controlled valves are capable of automatically controlling, in a first setting, a flow of the sample or one or more fractions between each of the three or more Modular Microfluidic Components, and in a second setting, unwanted materials to one of the plurality of waste systems.
6. A method as in claim 5 wherein said sample is a biofluid.
7. A method as in claim 6 wherein said biofluid is a human bodily fluid.
8. A method as in claim 5 further comprising at least one device for performing analysis interconnected to at least one or said output ports.
9. A system as in claim 1 wherein at least one of said Modular Microfluidic Components is located between two chemically incompatible Modular Microfluidic Components capable of performing buffer exchange resulting in chemical compatibility of said chemically incompatible Modular Microfluidic Components.
10. A method as in claim 5 wherein at least one of said Modular Microfluidic Components is located between two chemically incompatible Modular Microfluidic Components and performed buffer exchange resulting in chemical compatibility of said chemically incompatible Modular Microfluidic Components.