Transplantation of neural cells
Restoration or increase of inhibitory interneuron function in vivo is achieved by transplantation of MGE cells into the brain. Compositions containing MGE cells are provided as are uses to treat various diseases characterised by abnormal inhibitory interneuron function or in cases where increase inhibition may ameliorate neural circuits that are abnormally activated.
1. A method of treating a mammal afflicted with a seizure disorder, the method comprising:
transplanting medial ganglionic eminence (MGE) cells into the central nervous system of a mammal afflicted with a seizure disorder; and
allowing the transplanted cells to migrate and integrate in the central nervous system of said mammal to form functional inhibitory interneurons that are associated with a reduction in seizure activity in said mammal,
thereby treating the seizure disorder in said mammal.
2. The method of claim 1 , wherein the MGE cells are transplanted into the brain of said mammal.
3. The method of claim 1 , wherein the mammal is selected from the group consisting of mouse, rat, human, livestock animal and domestic animal.
4. The method of claim 1 , wherein the MGE cells are transplanted into a region of the central nervous system selected from the group consisting of cerebral cortex, hippocampus, thalamus, and striatum.
5. The method of claim 4 , wherein the MGE cells are transplanted into a region of the central nervous system which is free of lesions.
6. The method of claim 1 , wherein said transplanting comprises injecting dissociated MGE cells into the central nervous system.
7. The method of claim 6 , wherein the MGE cells are injected in association with a carrier.
8. The method of claim 1 , wherein the mammal is a human.
9. The method of claim 1 , wherein the mammal is an adult.
10. The method of claim 9 , wherein the mammal is a human.
11. The method of claim 10 , wherein said transplanting comprises injecting dissociated MGE cells into the central nervous system.
12. The method of claim 10 , wherein the MGE cells are transplanted into a region of the central nervous system selected from the group consisting of cerebral cortex, hippocampus, thalamus, and striatum.
13. The method of claim 1 , wherein said seizure disorder is epilepsy.
14. The method of claim 1 , wherein the reduction in seizure activity is measured by electroencephalography (EEG).
15. The method of claim 1 , wherein the reduction in seizure activity is measured by analysis of behavior.
16. The method of claim 1 , wherein the transplanted MGE cells cause a net increase in inhibitory neuron function in the brain relative to excitatory neuron function.
17. The method of claim 12 , wherein said seizure disorder is epilepsy.
18. The method of claim 17 , wherein the reduction in seizure activity is measured by electroencephalography (EEG).
19. The method of claim 17 , wherein the reduction in seizure activity is measured by analysis of behavior.