Nano-particle surface modification
Oxide, oxysulfide, or phosphate host particles with a self-assembled organo-phosphonate monolayer covalently bonded thereto are disclosed. Methods for coating the host particles and use of rare earth ion-doped particles in imaging methods and photodynamic therapy methods are also disclosed.
1. Particles comprising oxide, oxysulfide, or phosphate host particles with a self-assembled monolayer consisting of organophosphonate ligands covalently bonded thereto, wherein said host particles comprise ions of one or more rare earth elements, wherein said covalent bonding to the host particle surfaces is via tridentate phosphonate coordination, wherein said particles are less than about 100 microns in size, and wherein said organophosphonate ligands are of formula:
R—P(═O)(—OH) 2
wherein R is selected from the group consisting of linear alkyl, branched alkyl, linear alkylenyl, and branched alkylenyl moieties having from about two to about 40 carbon atoms, 0 to about 20 heteroatoms selected independently from oxygen and nitrogen, and omega-functionalized organic moieties, and wherein a photosensitizer compound or one or more biomolecular probes are covalently attached via said omega-functionalized organic moieties.
2. The particles of claim 1 , wherein the rare earth element ions are ions of one or more rare earth elements selected from the group consisting of strontium, europium, cerium, terbium, dysprosium, holmium, erbium, thulium, ytterbium and lutetium.
3. The particles of claim 2 , wherein said rare earth element ions comprise a combination of ytterbium ions and one or more ions selected from the group consisting of erbium, holmium and thulium ions.
4. The particles of claim 1 , wherein said host particles comprise oxides or oxysulfides of one or more elements selected from the group consisting of lanthanum, yttrium, lead, zinc, cadmium, beryllium, magnesium, calcium, strontium, barium, aluminum, radium, silicon and germanium.
5. The particles of claim 1 , wherein said host particles comprise oxides or oxysulfides of one or more Group II-IV semiconductor compounds.
6. The particles of claim 1 , wherein said host particles comprise one or more compounds selected from the group consisting of yttrium oxide (Y 2 O 3 ), lanthanum oxysulfide (La 2 O 2 S), yttrium oxysulfide (Y 2 O 2 S), calcium tungstate (CaWO 4 ) and gadolinium oxysulfide (Gd 2 O 2 S).
7. The particles of claim 6 , wherein said host particles comprise one or more compounds selected from the group consisting of yttrium oxide doped with erbium ions (Y 2 O 3 :Er), and yttrium oxide doped with ytterbium ions (Y 2 O 3 :Yb).
8. The particles of claim 1 , wherein the R groups of said organophosphonate ligands comprise poly(alkylene oxide) moieties.
9. The particles of claim 8 , wherein said poly(alkylene oxide) moieties comprise tetraethylene glycols with omega-functionalized organic moieties.
10. The particles of claim 1 , wherein the R groups of said organophosphonate ligands comprise butyl, hexyl, octyl, dodecyl, octadecyl, or undecyl moieties.
11. The particles of claim 1 , wherein said monolayer is more hydrophilic than the native surface of the underlying host particles.
12. A stable liquid suspension comprising the coated particles of claim 11 dispersed in a polar solvent.
13. A stable liquid suspension comprising a dispersion in a non-polar solvent of the particles of claim 11 encapsulated in micelles capable of suspending hydrophilic particles in a non-polar solvent or in cyclodextrins or liposomes with hydrophilic cores and hydrophobic exteriors.
14. The particles of claim 1 , wherein said monolayer is more hydrophobic than the native surface of the underlying host particles.
15. A stable liquid suspension comprising the particles of claim 14 dispersed in a non-polar solvent.
16. A stable liquid suspension comprising a dispersion in a polar solvent of the particles of claim 14 encapsulated in micelles capable of suspending hydrophobic particles in a polar solvent or in cyclodextrins or liposomes with hydrophobic cores and hydrophilic exteriors.
17. The particles of claim 1 , wherein said biomolecular probes are selected from the group consisting of proteins and peptides that bind to biological markers.
18. The particles of claim 17 , wherein said proteins are selected from the group consisting of avidin and antibodies.
19. The particles of claim 1 , wherein a photosensitizer compound is covalently attached via said omega-functionalized organic moieties.
20. The particles of claim 1 , wherein said particles are mono-disperse and have a particle size less than 1 micron.
21. The particles of claim 20 , wherein said particles have a particle size less than 100 nanometers.