Bio-sensing nanodevice
The invention provides a bio-sensing nanodevice comprising: a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—on a support, a real time receptor-ligand binding detection method, an odorant delivery system and an odorant recognition program. The biologically-derived G-protein coupled receptor can be stabilized on nanotechnology using surfactant peptide. The said nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor.
1. A bio-sensing nanodevice comprising: a stabilized biologically-derived G-protein coupled olfactory receptor-immobilized on a support, a real time receptor-ligand binding detection system method, a test composition delivery system and a test composition recognition program, wherein the stabilized receptor is stabilized with surfactant peptides selected from the group consisting of:
Sequence (N → C)
Formula
(φ) m (+) n
1
(+) n (φ) m
2
(φ) m (−) n
3
(−) n (φ) m
4
(−) n (φ) m (−) n
5
(+) n (φ) m (+) n
6
(φ) m (−)n(φ) m
7
(φ) m (+)n(φ) m
8
(+) n (φ) m (−) n
9
(−) n (φ) m (+) n
10
wherein:
(Φ) represents independently for each occurrence a natural or non-natural amino acid comprising a hydrophobic sidechain;
(+) represents independently for each occurrence a natural or non-natural amino acid comprising a sidechain that is cationic at physiological pH;
(−) represents independently for each occurrence a natural or non-natural amino acid comprising a sidechain that is anionic at physiological pH;
wherein the terminal amino acids are optionally substituted;
m for each occurrence represents an integer greater than or equal to 5; and
n for each occurrence represents an integer greater than or equal to 1;
wherein the test composition delivery system is a microfluidic bubble logic operation.
2. The bio-sensing nanodevice of claim 1 , wherein the microfluidic bubble logic operation comprises microfluidic channels adapted for receiving micron-sized droplets and bubbles comprising a test composition.