Porous cellulose gel, method for producing the same and use thereof
The present invention provides a porous cellulose gel having a high mechanical strength capable of being operated at a higher flow rate, and a method for producing the same. To a suspension liquid of cellulose particles, a crosslinking agent in an amount of from 4 to 12 times the amount of the cellulose monomer in terms of moles and an alkali in an amount of from 0.1 to 1.5 times the amount of the crosslinking agent in terms of moles are added continuously dropwise or added dividedly over a prescribed period of time, whereby flow rate characteristics of a resulting porous cellulose gel can be enhanced. According to the invention, the production efficiency of a polymer substance, such as a nucleic acid and a protein can be enhanced.
1. A method for producing porous crosslinked cellulose particles comprising the steps of:
adding continuously dropwise or adding dividedly, to a suspension liquid of non-crosslinked spherical cellulose particles having a sphericity of from approximately 0.8 to approximately 1.0, a crosslinking agent in an amount of from 4 to 12 times an amount of a cellulose monomer in terms of moles and an alkali in an amount of from 0.1 to 1.5 times the amount of the crosslinking agent in terms of moles, in the presence of at least one inorganic salt selected from the group consisting of a hydrochloride, a sulfate, a phosphate and a borate, in an amount of from 6 to 20 times the amount of the cellulose monomer in terms of moles, over 3 hours or more, and
producing crosslinked cellulose particles.
2. The method of claim 1 , wherein the amount of the crosslinking agent used is from 4 to 9 times the amount of the cellulose monomer in terms of moles.
3. The method of claim 1 , wherein the total amount of the crosslinking agent and the alkali used is divided into n portions (wherein n is an integer of from 2 to 4), and the adding step is repeated n times for each portion, to provide the porous cellulose gel of claims 1 .
4. The method as claimed in of claim 3 , wherein the total amount of the crosslinking agent used is from 6 to 12 times the amount of the cellulose monomer in terms of moles.
5. The method of claim 1 , wherein the suspension liquid of non-crosslinked cellulose particles has an initial alkali concentration of 1% by weight or less.
6. The method of claim 1 , wherein the total amount of the crosslinking agent and the alkali used is divided into n portions (wherein n is an integer of from 2 to 4), and the adding step is repeated n times for each portion, wherein the porous cellulose gel comprises crosslinked cellulose particles having a solvent contained therein, upon filling the gel in a chromatography column having an inner diameter of 2.2 cm to a height of 17.5±2.5 cm, a maximum linear velocity of water at 20° C. being from 2,400 to 5,500 cm/hour.
7. The method of claim 6 , wherein the total amount of the crosslinking agent used is from 6 to 12 times the amount of the cellulose monomer in terms of moles.
8. The method of claim 1 , wherein the total amount of the crosslinking agent and the alkali used is divided into n portions (wherein n is an integer of from 5 to 10), and the adding step is repeated n times for each portion, to provide the porous cellulose gel of claim 1 .