USE OF NEUREGULIN FOR ORGAN PRESERVATION
The present invention provides compositions and methods for preservation, perfusion or reperfusion of an organ, such as heart, for transplantation. The compositions comprise a preservation solution and an effective amount of neuregulin. The methods comprising contacting an organ with an effective amount of neuregulin.
1 . A composition for perfusion, preservation or reperfusion of an organ for transplantation, comprising a preservation solution and an effective amount of neuregulin.
2 . The composition of claim 1 , wherein the preservation solution is selected from the group consisting of Celsior solution, Krebs-Henseleit solution, normal saline solution, University of Wisconsin solution, St. Thomas II solution, Collins solution, and Stanford solution.
3 . The composition of claim 1 , wherein the preservation solution is Celsior solution.
4 . The composition of claim 1 , wherein the preservation solution is Krebs-Henseleit solution.
5 . The composition of claim 1 , wherein neuregulin comprises the amino acid sequence of SEQ ID NO:1.
6 . The composition of claim 1 , wherein neuregulin is at the concentration of about 10-20 nM.
7 . The composition of claim 1 , wherein neuregulin is at the concentration of about 14 nM.
8 . A method for preserving an organ for transplantation, said method comprising contacting the organ with an effective amount of neuregulin.
9 . The method of claim 8 , wherein the organ is heart.
10 . The method of claim 8 , wherein The composition of claim 1 , wherein neuregulin comprises the amino acid sequence of SEQ ID NO:1.
11 . The method of claim 8 , wherein neuregulin is administered in an amount sufficient to activate AKT signaling pathway.
12 . A method for increasing the relaxation speed of heart in a subject, said method comprising administering an effective amount of neuregulin to a subject.
13 . The method of claim 12 , wherein the subject is human.
14 . A method for treating chronic heart failure in a subject, comprising administering an effective amount of neuregulin for six hours to a subject in need thereof.
15 . The method of claim 14 , wherein the subject is human.
16 . The method of claim 14 , wherein neuregulin improve cardiac output in the subject.
17 . The method of claim 14 , wherein neuregulin lower systemic vascular resistance in the subject.