Antigen delivery vectors and constructs
The present invention relates to fluorocarbon vectors for the delivery of antigens to immunoresponsive target cells. It further relates to fluorocarbon vector-antigen constructs and the use of such vectors associated with antigens as vaccines and immunotherapeutics in animals.
1. A method of treating a subject in need of immunization comprising the step of administering an effective amount of a fluorocarbon vector-antigen construct of structure C m F n —C y H x -(Sp)-R, where m=3 to 30, n<=2 m+1, y=0 to 15, x<=2y, (m+y)=3-30, Sp is an optional chemical spacer moiety, and R is an immunogenic peptide.
2. A method of stimulating an immune response in a subject in need thereof comprising the step of administering an effective amount of a fluorocarbon vector-antigen construct of structure C m F n —C y H x -(Sp)-R, where m=3 to 30, n<=2 m+1, y=0 to 15, x<=2y, (m+y)=3-30, Sp is an optional chemical spacer moiety, and R is an immunogenic peptide.
3. The method of claim 2 wherein the subject is a mammal.
4. The method of claim 1 wherein the construct is combined with antiviral therapy.
5. The method of claim 2 wherein the construct is combined with antiviral therapy.
6. The method of claim 1 wherein the subject is a mammal.
7. The method of claim 1 wherein the fluorocarbon vector-antigen construct structure is
8. The method of claim 1 wherein the fluorocarbon vector-antigen construct structure is
9. The method of claim 1 wherein the fluorocarbon vector-antigen construct structure is
10. The method of claim 1 wherein R is an antigen from a virus, bacteria, parasite, an autologous protein or cancer antigen.
11. The method of claim 1 wherein R comprises one or more epitopes from a viral protein.
12. The method of claim 1 wherein R is a peptide consisting of between 7 to 70 amino acids.
13. The method of claim 2 wherein the fluorocarbon vector-antigen construct structure is
14. The method of claim 2 wherein the fluorocarbon vector-antigen construct structure is
15. The method of claim 2 wherein the fluorocarbon vector-antigen construct structure is
16. The method of claim 2 wherein R is an antigen from a virus, bacteria, parasite, an autologous protein or cancer antigen.
17. The method of claim 2 wherein R comprises one or more epitopes from a viral protein.
18. The method of claim 2 wherein R is a peptide consisting of between 7 to 70 amino acids.
19. The method of claim 3 , wherein the mammal is a human.
20. The method of claim 6 , wherein the mammal is a human.
21. A method of lowering the risk of influenza in a human subject in need thereof comprising the step of administering an effective amount of a fluorocarbon vector-antigen construct of structure C m F n —C y H x -(Sp)-R, where m=3 to 30, n<=2 m+1, y=0 to 15, x<=2y, (m+y)=3-30, Sp is an optional chemical spacer moiety, and R is an immunogenic peptide comprising one or more epitopes from an influenza protein.
22. The method of claim 21 wherein R is a peptide consisting of between 7 to 70 amino acids.
23. The method of claim 21 wherein the fluorocarbon vector-antigen construct structure is
24. The method of claim 21 wherein the fluorocarbon vector-antigen construct structure is
25. The method of claim 21 wherein the fluorocarbon vector-antigen construct structure is