Microfluidic devices and methods
Disclosed herein are devices and methods useful for the detection and analysis of various analytes. The analyte detection device of this invention has the general structure of a substrate and a plurality of pillars attached to the surface of the substrate.
1. An analyte detection device comprising a substrate and a plurality of pillars attached to the surface of the substrate, wherein each pillar comprises a sample surface and side surfaces, each of the side surfaces perpendicular to the surface of the substrate, and wherein the sample surface of each pillar and the surface of the substrate have the same coating, and wherein each pillar comprises a porous material.
2. The device of claim 1 , wherein the side surfaces of each pillar, the sample surface of each pillar, and the surface of the substrate have the same coating.
3. The device of claim 1 , further comprising an interlayer between the sample surfaces and the coating on the sample surfaces.
4. The device of claim 1 , further comprising an interlayer between the substrate surface and the coating on the substrate surface.
5. The device of claim 3 or 4 , wherein the interlayer comprises silicon oxide.
6. The device of claim 1 or 2 , wherein the coating further comprises an affinity structure.
7. The device of claim 6 , wherein the affinity structure comprises a monolayer of an organic molecule.
8. The device of claim 7 , wherein the organic molecule is polylysine or polyethylene glycol.
9. The device of claim 6 , wherein the affinity structure comprises a capture agent.
10. The device of claim 6 , wherein the capture agent comprises an antibody, antibody fragment, polypeptide, receptor, DNA strand or fragment, RNA strand or fragment, or aptamer.
11. The device of claim 6 , wherein the affinity structure comprises biotin, avidin, or streptavidin.
12. The device of claim 1 , wherein the substrate and plurality of pillars are made of the same material.
13. The device of claim 1 , wherein the substrate and plurality of pillars are made of different materials.
14. The device of claim 1 , wherein the plurality of pillars are made of a porous material.
15. The device of claim 1 , wherein the sample surface of each pillar is located distal to the substrate surface.
16. The device of claim 1 , wherein the plurality of pillars are substantially perpendicular with respect to the surface of the substrate.
17. The device of claim 1 , wherein the plurality of pillars range in height from about 1 to 200 microns.
18. The device of claim 1 , wherein the plurality of pillars are arranged in a regular array.
19. The device of claim 1 , wherein the plurality of pillars are arranged in an irregular array.
20. The device of claim 1 , wherein the density of the plurality of pillars is about 25 pillars/cm 2 or greater.
21. The device of claim 1 , wherein the number of pillars per device is greater than 10, 100, or 1000 pillars.
22. The device of claim 1 , wherein the pillar pitch is 500 microns or less.
23. The device of claim 1 , wherein the sample surface of each pillar or coating of each pillar binds an analyte.
24. The device of claim 1 , wherein the analyte is selected from the group consisting of pharmaceuticals, metabolites, pesticides, polypeptides, proteins, DNA, and RNA.