Immobilized proteins and methods and uses thereof
The invention relates to the field of covalently attaching proteins to a substrate, particularly to methods of immobilizing proteins by posttranslationally modifying a cysteine residue of said protein through the addition of functional groups. The invention also relates to biological molecules used in such techniques, including proteins, and detection methods and kits that utilize such immobilized proteins, such as a microdevice or “protein chip”, a high-throughput screening device, and for the microscopy of proteins on a surface.
1. An isolated soluble protein comprising a non-native C-terminal CaaX motif, wherein C is Cys, “a” is any amino acid and X is selected from the group consisting of Ala, Ser, Met, Leu and Gln, in which the cysteine has been posttranslationally modified by the addition of a functional group, wherein said protein has been immobilized to a substrate comprising an azide-derivatized surface or an alkyne-derivatized surface by ligating the functional group to said substrate surface, and wherein a native fold configuration of said protein is preserved during and after protein immobilization.
2. The protein of claim 1 , where X permits alkylation of the cysteine residue by analogues of farnesyl diphosphate or geranylgeranyl diphosphate.
3. The protein of claim 1 , wherein said posttranslational modification of a cysteine includes alkylation of the sulthydryl moiety comprising one or more of catalysis of a farnesyl analog with protein farnesyltransferase and catalysis of a geranylgeranyl analog with protein geranylgeranyltransferase.
4. The protein of claim 1 , where said functional group comprises one or more of an azide and an alkyne.
5. The protein of claim 3 , wherein said farnesyl analog is selected from analogs depicted in FIG. 9 .
6. The protein of claim 1 , wherein said substrate comprises one or more of a silicon surface selected from the group consisting of silicon, glass, SiO 2 , and silicon nitride, a polymer surface comprising one or more of polymer beads and polymer films, a gel surface comprising one or more of an agarose gel and an acrylamide gel, and a metal surface comprising one or more of platinum, gold, silver, copper, zinc sulfide, cadmium selenide, zinc sulfide-capped cadmium selenide, titanium dioxide, aluminum, aluminum oxide, opal films, and ceramics.
7. The protein of claim 1 , wherein said substrate comprises one or more of an azide-derivatized glass surface and an alkyne-derivatized glass surface.
8. The protein of claim 5 , wherein the farnesyl analog is
9. The protein of claim 5 , wherein the farnesyl analog is
10. The protein of claim 5 , wherein the farnesyl analog is
where n =0, 1, or 2.
11. The protein of claim 5 , wherein the farnesyl analog is
where n =1 or 3.
12. The protein of claim 5 , wherein the farnesyl analog is