Microfluidic device for controlled aggregation of spider silk
The present invention is directed to a device and method of controlling the phase separation of one or a mixture of two or more spider silk proteins, leading to the defined and controllable assembly of the said silk protein(s) to defined morphologies, such as spheres, nano fibrils, threads, etc.
1. A microfluidic device comprising:
a) at least three microfluidic channels formed in said device comprising fluids flowing therethrough, wherein the channels converge in said microfluidic device to one combined channel in order to provide a laminar flow of said fluids, said combined channel leading to an outlet port;
b) at least three inlet ports formed in said device to permit access to the at least three microfluidic channels; and
c) at least three fluid storage means, each being connected via said inlet ports to one of the at least three microfluidic channels, characterized in that at least one of said storage means comprises a solution of one or more spider silk proteins and at least one of said storage means comprises an ionic solution comprising potassium and phosphate ions.
2. The microfluidic device of claim 1 , wherein the solution of one or more spider silk proteins comprises authentic or synthetic spider silk proteins.
3. The microfluidic device of claim 1 , which additionally comprises a storage means connected to a microfluidic channel, comprising a solution of one or more substances selected from the group consisting of polypeptides, polysaccharides, marker molecules, quantum dots, metals, nucleic acids, lipids and low molecular drugs.
4. The microfluidic device of claim 1 , wherein the diameter of the microfluidic channels is narrowing in the direction from inlet to outlet port.
5. A method for assembling spider silk, comprising the steps of:
a) providing a microfluidic device according to claim 1 ;
b) controllably flowing fluids through the microfluidic channels by suitable means, thereby providing a laminar flow of said fluids after converging of said microfluidic channels; and thereby
c) aggregating said spider silk proteins.
6. The method of claim 5 , wherein the spider silk proteins are modified by substances selected from the group consisting of polypeptides, polysaccharides, marker molecules, quantum dots, metals, nucleic acids, lipids and low molecular drugs.