High-throughput RNA structure analysis
View Patent ↗The presently disclosed subject matter relates to technology and methods for analyzing the structure of RNA molecules. More particularly, the presently disclosed subject matter is directed to methods of, compositions for, and computer program products for RNA structure analysis through alkoxide-selective 2′-hydroxyl acylation analyzed by primer extension.
1. A method of forming a covalent ribose 2′-O-adduct within an RNA molecule, the method comprising contacting an electrophile with RNA wherein the electrophile selectively modifies unconstrained nucleotides in the RNA to form a covalent ribose 2′-O-adduct, wherein the electrophile is the isatoic anhydride derivative 1-methyl-7-nitroisatoic anhydride (1M7).
2. A method of forming a covalent ribose 2′-O-adduct within an RNA molecule, the method comprising contacting an electrophile with RNA wherein the electrophile selectively modifies unconstrained nucleotides in the RNA to form a covalent ribose 2′-O-adduct, wherein the electrophile is the phthalic anhydride derivative 4 nitrophthalic anhydride (4NPA).
3. The method of claim 1 or claim 2 , wherein the RNA is present in a biological solution.
4. A covalent ribose 2′-O-adduct within an RNA molecule formed by the method of claim 1 .
5. A covalent ribose 2′-O-adduct, comprising RNA and an electrophile bound at the 2′-O position of one or more unconstrained nucleotides in the RNA, wherein the electrophile is selected from the group consisting of 1M7 and 4NPA.