Method for the detection of sepsis using procalcitonin-3-116
Uses of recombinant procalcitonin 3-116 in the diagnosis and therapy of septic diseases and the measurement of prohormones other than procalcitonin, and of dipeptidyl peptidase IV, as biomarkers in the diagnosis of sepsis.
1. A method for the detection of sepsis in an individual suspected of having sepsis, comprising:
isolating procalcitonin 3-116 from a plasma or serum sample from the individual by contacting with a procalcitonin antibody bound to a support material suitable as a material for an affinity column,
washing said material to retain bound procalcitonin 3-116 peptide, and
determining the level of procalcitonin 3-116, wherein an increased level of procalcitonin 3-116 compared to healthy individuals is indicative of sepsis.
2. A method of claim 1 wherein said material is a beaded material.
3. A method of claim 1 wherein said material is beaded agarose.
4. A method of claim 1 wherein said material comprises magnetic beads.
5. A method for the detection of sepsis in an individual suspected of having sepsis, comprising:
contacting a plasma or serum sample from the individual with a procalcitonin antibody bound to a support material suitable as a material for an affinity column,
washing said material to retain bound procalcitonin 3-116 peptide, and
determining the level of procalcitonin 3-116, wherein an increased level of procalcitonin 3-116 compared to healthy individuals is indicative of sepsis.
6. A method of claim 5 wherein said material is a beaded material.
7. A method of claim 5 wherein said material is beaded agarose.
8. A method for the detection of sepsis in an individual suspected of having sepsis, comprising:
isolating procalcitonin 3-116 from a plasma or serum sample from the individual by contacting with a procalcitonin antibody bound to an affinity matrix,
washing said material to retain bound procalcitonin 3-116 peptide, and
determining the level of procalcitonin 3-116, wherein an increased level of procalcitonin 3-116 compared to healthy individuals is indicative of sepsis.
9. A method for the detection of sepsis in an individual suspected of having sepsis, comprising:
contacting a plasma or serum sample from the individual with a procalcitonin antibody bound to a support material suitable as a material for affinity isolation,
washing said material to retain bound procalcitonin 3-116 peptide, and
determining the level of procalcitonin 3-116, wherein an increased level of procalcitonin 3-116 compared to healthy individuals is indicative of sepsis.