Filovirus vectors and noninfectious filovirus-based particles
Cloned filovirus genomic cDNA and methods of using the cDNA are provided. Further provided are noninfectious lipid encapsulated filovirus-based particles.
1. A method to prepare a vaccine comprising filamentous filovirus-like particles, comprising: isolating filamentous filovirus-like particles from a mammalian cell contacted with a vector comprising a promoter operably linked to a DNA segment encoding a filovirus matrix protein and a vector comprising a promoter operably linked to a DNA segment encoding a filovirus glycoprotein; and preparing a vaccine comprising an amount of the isolated filamentous filovirus-like particles effective to induce an immune response.
2. The method of claim 1 wherein the cell is further contacted with a vector comprising a promoter operably linked to a DNA segment encoding a protein of interest.
3. The method of claim 1 wherein the filovirus matrix protein is an Ebola virus matrix protein.
4. A method to prepare a vaccine comprising filamentous filovirus-like particles, comprising: isolating filamentous filovirus-like particles from a mammalian cell contacted with a vector comprising a promoter operably linked to a DNA segment encoding a filovirus matrix protein and a vector comprising a promoter operably linked to a DNA segment encoding a filovirus glycoprotein; and preparing a vaccine comprising an amount of the isolated filamentous filovirus-like particles effective to induce an immune response, wherein the glycoprotein is a heterologous filovirus glycoprotein.
5. The method of claim 1 wherein the filovirus matrix protein comprises the amino acid motif PPAY at positions corresponding to positions 10 to 13 of SEQ ID NO:3.
6. A vaccine comprising the isolated filovirus-like particles prepared by the method of claim 1 .
7. A vaccine comprising isolated filovirus-like particles comprising filovirus matrix protein and filovirus glycoprotein.