Mouse model of retinal degeneration
The invention is directed to a method of producing a non-human mammal having one or more pathological characteristics of retinal degeneration and/or age-related macular degeneration. In particular, the invention provides a method of producing a non-human mammal having age-related macular degeneration (AMD). The invention is also directed to non-human animals produced by the methods described herein. Methods of identifying an agent for use in inhibiting one or more pathological characteristics of retinal degeneration and/or AMD is also encompassed by the invention. Also provided is a method of treating AMD in an individual in need thereof comprising, administering to the individual an agent identified herein.
1. A method of producing a mouse having one or more pathological characteristics of retinal degeneration comprising:
a) subcutaneously administering to a mouse a composition comprising an adjuvant and a carboxyethylpyrrole (CEP) adducted mouse serum albumin to produce antibodies against CEP; and
b) maintaining the mouse of step (a) under conditions in which one or more pathological characteristics of retinal degeneration develops in said mouse, wherein one or more pathological characteristics of retinal degeneration is selected from the group consisting of lysis of retinal pigmented epithelium (RPE) cells, the presence of invading inflammatory cell in the interphotoreceptor matrix (IPM), and focal loss of RPE,
thereby producing mouse having one or more pathological characteristics of retinal degeneration.
2. The method of claim 1 , wherein the mouse is administered multiple doses of the composition.
3. The method of claim 2 , wherein the mouse is administered the composition on day 0, day 10 and day 60.
4. The method of claim 2 , wherein the mouse is administered the composition on day 0 and on day 10.
5. The method of claim 1 , wherein the adjuvant is selected from the group consisting of: complete Freund's adjuvant (CFA) and incomplete Freund's adjuvant (IFA).
6. The method of claim 5 , wherein the adjuvant is complete Freund's adjuvant (CFA) and incomplete Freund's adjuvant (IFA).
7. The method of claim 5 , wherein the adjuvant is complete Freund's adjuvant (CFA).