Methods to identify protein arginine deiminase 4 inhibitors
In accordance with one embodiment of the present disclosure, a method to identify a protein arginine deiminase 4 inhibitor is disclosed. The method includes performing a competitive assay in which a potential inhibitor compound competes with rhodamine-conjugated fluoroamidine to bind to protein arginine deiminase 4. Fluorescence is measured to determine an estimate of the amount of fluorescent protein arginine deiminase 4 that is present in the assay.
1. A method to identify a protein arginine deiminase 4 inhibitor comprising:
performing a control assay in which rhodamine-conjugated fluoroamidine binds to protein arginine deiminase 4;
measuring fluorescence to determine an estimate of the amount of fluorescent protein arginine deiminase 4 that is present in the control assay;
performing a competitive assay in which a potential inhibitor compound competes with rhodamine-conjugated fluoroamidine to bind to protein arginine deiminase 4; and
measuring fluorescence to determine an estimate of the amount of fluorescent protein arginine deiminase 4 that is present in the competitive assay;
determining if the potential inhibitor compound is likely to act as an inhibitor of protein arginine deiminase 4 based on the estimate of the amount of fluorescent protein arginine deiminase 4 that is present in the competitive assay, wherein a decrease in fluorescence in the competitive assay when compared to the control assay is indicative that the potential inhibitor compound is a protein arginine deiminase 4 inhibitor.
2. A method as in claim 1 , wherein no decrease in the fluorescence in the competitive assay when compared to the control assay is indicative that the potential inhibitor compound is not a protein arginine deiminase 4 inhibitor.
3. A method as in claim 1 , wherein the potential inhibitor compound comprises a disease modifying anti-rheumatic drug.
4. A method as in claim 3 , wherein the potential inhibitor compound comprises a chemical disease modifying anti-rheumatic drug.