Process of Producing Ethanol Using Cellulose with Enzymes Generated Through Solid State Culture

1 . A method of producing ethanol, comprising:

(1) providing an SSC Cellulase enzyme composition made by a solid state culture process, said process comprising:

(i) steaming a substrate comprising cob, straw, barley, and wheat germ;

(ii) growing a fungus on said substrate for a first period of time in a growth chamber; and

(iii) harvesting the enzyme composition;

(2) providing a mash that is adjusted to pH 4 to 6;

(3) mixing said mash with said SSC Cellulase enzyme composition and yeast; and

(4) incubating for a period of fermentation time under a temperature between 20 to 40° C. to produce ethanol.

2 . The method according to claim 1 , wherein said fungus is the strain of Trichoderma reesei , ATCC-57560.

3 . The method according to claim 1 , wherein said method further comprising collecting said ethanol.

4 . The method according to claim 3 , further comprises distilling said ethanol.

5 . The method according to claim 1 , wherein said mash has up to 40% solid.

6 . The method according to claim 1 , wherein said mash is adjusted to pH about 4.8.

7 . The method according to claim 1 , wherein said temperature is about 35° C.

8 . The method according to claim 1 , wherein said mash comprises cellulose material with lignin, and wherein said cellulose is pretreated using alkaline processes to remove said lignin.

9 . The method according to claim 1 , wherein said incubating step comprises simultaneously hydrolysis and fermentation.

10 . The method according to claim 1 , wherein said fungus is selected from a group consisting of T. reesei, Aspergillus niger, A. phoenicis, A oryzae, A awamori, Rhizopus oryzae, R. microsporus, Acidothermus cellulyticus and Trichoderma koningii, Trichoderma viride. T. harzianum, Fusarium oxysporum Penicillum pupurogenum , and Myceliophthora sp., Lentinous.

11 . A method of making enzyme composition comprising.

(1) providing a solid fermentation substrate; and

(2) growing a Trichoderma reesei ATCC-57560 on said substrate for a first period of time in a growth chamber to produce an enzyme composition.

12 . The method according to claim 11 , further comprising harvesting said enzyme composition.

13 . The method according to claim 11 , wherein said substrate comprises barley.

14 . The method according to claim 11 , wherein said substrate has undergone heat treatment.

15 . The method according to claim 14 , wherein said heat treatment is steaming.

16 . The method according to claim 15 , wherein said steaming is conducted at ambient pressure.

17 . A composition comprising:

(1) a Trichoderma reesei ATCC-57560; and

(2) a substrate comprises corn cob, straw, barley, and wheat germ; and

(3) endo and exo acting cellulase, cellobiase, and xylanses.

18 . The composition according to claim 17 , wherein said Trichoderma reesei produces endo and exo acting cellulase, cellobiase, and xylanses at a pH optima of 4.8.

19 . A composition comprising:

(1) an SSC Cellulase enzyme composition;

(2) a yeast; and

(3) mash.

20 . The composition according to claim 19 , wherein said SSC Cellulase enzyme composition is made by a solid state culture process, said process comprising:

(i) steaming a substrate to adjust moisture and reduce contamination from indigenous microorganisms, wherein said substrate comprises cob, straw, barley, and wheat germ;

(ii) growing a Trichoderma reesei , ATCC-57560 on said substrate for a first period of time in a growth chamber; and

(iii) harvesting the enzyme mush comprises the mixture of said Trichoderma reesei and substrate.