Chromatography devices and methods
View Patent ↗Provided are chromatography devices having a stationary phase that includes patchy particles having at least two different surface chemistries, such as Janus particles. Also provided are methods of separating at least one analyte out of a sample, where the method includes adding a sample having at least one analyte to a chromatography device that includes a stationary phase that includes a plurality of patchy particles. Further provided are methods that include packing a chromatography column with a plurality patchy particles having at least two different surface chemistries. Additional embodiments are disclosed.
1. A chromatography device comprising a stationary phase comprising a plurality of silica Janus particles, said silica Janus particles comprising at least a hydrophobic first surface coated with at least one alkyl chain, and a hydrophilic second surface having no coating thereon.
2. The chromatography device of claim 1 , wherein the at least one alkyl chain is selected from the group consisting of: C 4 , C 8 and C 18 alkyl chains.
3. The chromatography device of claim 1 , wherein the chromatography device comprises a high performance liquid chromatography device.
4. The chromatography device of claim 1 , wherein said silica Janus particles are colloidal-sized particles.
5. The chromatography device of claim 1 , wherein said silica Janus particles comprise substantially spherical particles.
6. A chromatography device, comprising a stationary phase comprising a plurality of patchy particles, said patchy particles comprising at least a first surface and a second surface, the first surface having a different surface chemistry than the second surface.
7. The chromatography device of claim 6 , wherein said first surface is a modified particle surface.
8. The chromatography device of claim 7 , wherein said modified particle surface comprises a coating.
9. The chromatography device of claim 8 , wherein said coating comprises at least one hydrophobic substance.
10. The chromatography device of claim 9 , wherein said hydrophobic substance is an alkyl chain.
11. The chromatography device of claim 7 , wherein said modified particle surface is a result of applying electromagnetic radiation to the first surface.
12. The chromatography device of claim 6 , wherein said second surface is a modified particle surface.
13. The chromatography device of claim 6 , wherein said second surface is an unmodified particle surface.
14. The chromatography device of claim 6 , wherein said patchy particles comprise at least one material selected from the group consisting of polymers, polypeptides, proteins, nucleic acids, glass, ceramics, metals and chemical elements.
15. The chromatography device of claim 14 , wherein said patchy particles comprise fused silica.
16. The chromatography device of claim 6 , wherein said patchy particles comprise at least one shape selected from the group consisting of spherical, ellipsoid, rodlike, helical and oblate.
17. The chromatography device of claim 6 , wherein said first surface is hydrophobic and said second surface is hydrophilic.
18. The chromatography device of claim 6 , wherein said first surface comprises a positively charged 3-aminopropyltriethoxysilane (APS) and said second surface comprises non-polar octadecyltrichlorosilane (OTS).
19. The chromatography device of claim 6 , wherein said chromatography device is capable of at least one chromatographic separation method selected from the group consisting of: normal phase liquid chromatography, reverse phase liquid chromatography, ion exchange chromatography, size exclusion, high performance liquid chromatography, and affinity chromatography.
20. The chromatography device of claim 6 , wherein the plurality of patchy particles comprises a plurality of Janus particles.
21. A chromatography device, comprising a stationary phase comprising a plurality of patchy particles, said patchy particles comprising a plurality of surfaces, each surface having a different chemistry.