Micro fluid device and trace liquid diluting method
A microfluidic device provided with a micro-channel structure capable of easily and positively providing therein micro-droplet having various dilution ratios. A micro-channel structure provided in a substrate ( 2 ) has a first mixing unit ( 11 ) and a second mixing unit ( 21 ) connected to the downstream side of the first mixing unit ( 11 ), with each mixing unit ( 11, 21 ) having first through third micro-channels. One end of a first weighing unit ( 11 d ) consisting of a micro-channel having a capacity equivalent to the volume of a specified-amount first micro-droplet is opened to a first micro-channel ( 11 a ), and the other end is opened to a merging unit ( 12 a ) provided on a second micro-channel ( 12 ). One end of a second weighing unit ( 13 d ) consisting of a micro-channel having a capacity equivalent to the volume of a specified-amount second micro-droplet is connected to a third micro-channel ( 13 ), and the other end is opened to the merging unit ( 12 a ). Any one of the first through third outlet ports of the first mixing unit is connected with the first or the third inlet port of the second mixing unit ( 21 ).
1. A microfluidic device having
a substrate; and
a micro-channel structure through which a micro-droplet is transported, the micro-channel structure being formed in the substrate,
the micro-channel structure having a first mixing unit, and a second mixing unit connected to a downstream of the first mixing unit,
each mixing unit comprising:
a first measuring section consisting of a micro-channel having a capacity equal to a volume of a first micro-droplet having a fixed amount, for measuring of a fixed amount of the first micro-droplet;
a second measuring section consisting of a micro-channel having a capacity equal to a volume of a second micro-droplet of a fixed amount, for measuring a fixed amount of the second micro-droplet;
a merging section for merging the first and the second micro-droplets that have been measured in the first and the second measuring section;
a mixing section for mixing the first and the second micro-droplets, the mixing section connected in series to a downstream of the merging section;
an exhausting section for exhausting the mixed droplet obtained by mixing the first and the second micro-droplets;
a first to a third inlet ports and a first to a third outlet ports;
a first micro-channel for connecting the first inlet port and the first outlet port;
a second micro-channel having the merging section, the mixing section, and the exhausting section, the second micro-channel connecting the second inlet port and the second outlet port;
a third micro-channel for connecting the third inlet port and the third outlet port;
an end of the first measuring section being connected to the first micro-channel,
another end of the first measuring section having an opening in the merging section provided in the second micro-channel,
an end of the second measuring section being connected to the third micro-channel,
another end of the second measuring section having an opening in the merging section provided in the second micro-channel,
the second outlet port being connected to the exhausting section,
one of the outlet ports in the first to the third outlet ports of the first mixing unit being connected to the first or the third inlet port of the second mixing unit,
wherein the second outlet port of the first mixing unit is connected to and in fluid communication with the first inlet port of the second mixing unit such that liquid exiting the second outlet port enters the first inlet port of the second mixing unit.
2. The microfluidic device, according to claim 1 , wherein a micro-droplet mixed in the first mixing unit is used as a fixed amount of the first or the second micro-droplet in the second mixing unit.
3. The microfluidic device according to claim 2 , wherein the opening of the end of the first measuring section in the merging section provided in the second micro-channel and the opening of the end of the second measuring section in the merging section provided in the second micro-channel are disposed in different positions with each other in a flowing direction of a micro-droplet in the merging section in the first and/or the second mixing unit.
4. The microfluidic device according to claim 2 , wherein a distance between the opening of the end of the first measuring section in the merging section provided in the second micro-channel and the opening of the end of the second measuring section in the merging section provided in the second micro-channel, in the flowing direction of the micro-droplet in the second micro-channel is selected so as to avoid formation of an air bubble between the first micro-droplet supplied to the merging section from the first measuring section and the second micro-droplet supplied to the merging section from the second measuring section, and so as to avoid contact of the first and the second micro-droplet to the outlet opening of the second measuring section, or to the outlet opening of the first measuring section upon exhausting at different timings of the first and the second micro-droplet to the merging, section from the second measuring section.