Treatment of multiple sclerosis and/or rheumatoid arthritis
The invention relates to the use of an antibody which can specifically bind to chemokine receptor CCR2 for producing a medicament utilized for the treatment of multiple sclerosis and/or rheumatoid arthritis in a subject that is preferably a primate or a human. In another embodiment, the invention relates to the use of an antibody which can specifically bind to chemokine receptor CCR2 for producing a medicament that is utilized for depleting monocytes in subjects suffering from multiple sclerosis and/or rheumatoid arthritis. The invention further relates to corresponding in vitro methods and therapeutic methods. And antibody which binds to CD 14, for example, can be used in addition to the antibody that can bind to CCR2.
1. A medicament containing a monoclonal antibody which specifically binds to loop 1+first half of loop 2 of the extracellular human chemokine receptor CCR2 domain, wherein the monoclonal antibody is Doc-2.
2. A method for depleting monocytes comprising contacting the monocytes with a monoclonal antibody which specifically binds to loop 1+first half of loop 2 of the extracellular human chemokine receptor CCR2 domain, wherein the monoclonal antibody is Doc-2.
3. A method for depleting monocytes in a human subject comprising the step of administering to the subject an effective dose of a monoclonal antibody Doc-2 which specifically binds to loop 1+first half of loop 2 of extracellular human CCR2 domain.
4. The method according to claim 3 , wherein the subject has a disease selected from the group consisting of a therapy-refractory multiple sclerosis, a multiple sclerosis in an advanced stage, and a combination thereof.
5. The method according to claim 3 , wherein the effective dose does not exceed that dose which is required to achieve a depletion of 95% of the monocytes in the peripheral blood.
6. The method according to claim 5 , wherein the effective dose is at least 20% of the monocytes in the peripheral blood are depleted.
7. The method according to claim 3 , wherein the effective dose does not cause a more than 20-fold increase of the level of interleukin-6 (IL-6) and/or interleukin-4 (IL-4) in the blood plasma.
8. The method according to claim 3 , wherein the effective dose corresponds to 0.01-20 mg of the antibody per kg body weight of the subject per day.
9. A method for depleting monocytes in a human subject comprising the step of administering to the subject a monoclonal antibody which specifically binds to loop 1+first half of loop 2 of the extracellular human CCR2 domain, wherein the monoclonal antibody is Doc-2.
10. The method according to claim 9 , wherein the human subject has multiple sclerosis.
11. The method according to claim 3 , wherein the antibody is a humanized antibody.
12. The method according to claim 3 , wherein the antibody is an antibody of the IgG1 isotype.
13. The method according to claim 9 , wherein the antibody is a humanized antibody.
14. The method according to claim 9 , wherein the antibody is an antibody of the IgG1 isotype.
15. The method according to claim 3 , wherein the effective dose does not exceed that close which is required to achieve a depletion of 90% of the monocytes in the peripheral blood.
16. The method according to claim 3 , wherein the effective dose does not exceed that dose which is required to achieve a depletion of 80% of the monocytes in the peripheral blood.
17. The method according to claim 5 , wherein the effective dose is at least 30% of the monocytes in the peripheral blood are depleted.
18. The method according to claim 3 , wherein the effective dose does not cause a more than 10-fold increase of the level of interleukin-6 (IL-6) and/or interleukin-4 (IL-4) in the blood plasma.