Pathogen-inducible synthetic promoter
The invention relates to a pathogen-inducible synthetic promoter which is suitable for regulating the transcription of a nucleic acid, and includes a minimal promoter, characterized in that the minimal promoter includes a sequence motif a) dbrmwa or b) twcccmt which is disposed downstream from a TATA region and in front of a transcription starting point which is located on the minimal promoter and at which transcription of the nucleic acid to be regulated starts.
1. A pathogen inducible synthetic promoter for regulating the transcription of a nucleic acid, which includes at least one cis regulatory element and a minimal promoter,
wherein the minimal promoter is heterologous to the at least one cis regulatory element,
wherein the minimal promoter consists essentially of a TATA-box, a transcription starting point, and a sequence motif dbrmwa, and
wherein the sequence motif dbrmwa is disposed downstream from the TATA-box and in front of the transcription starting point at which transcription of the nucleic acid to be regulated starts.
2. The pathogen inducible synthetic promoter according to claim 1 , wherein the sequence motif occurs two or more times in the minimal promoter.
3. The pathogen inducible synthetic promoter according to claim 1 , in which the minimal promoter includes the nucleotide sequence according to SEQ ID NO: 2 or derivatives thereof.
4. The pathogen inducible synthetic promoter according to claim 1 , which includes in addition to the minimal promoter at least one cis-regulating element with the nucleotide sequence according to SEQ ID NO: 13.
5. A recombinant gene comprising the pathogen inducible synthetic promoter according to claim 1 .
6. A plant cell, in which the pathogen inducible synthetic promoter according to claim 1 has been integrated into DNA of the plant cell.
7. A transgenic plant with a plant cell according to claim 6 .
8. A seed of a transgenic plant comprising the pathogen inducible synthetic promoter according to claim 1 .
9. A process for producing a pathogen resistant plant, comprising introducing into a plant cell a nucleic acid causing pathogen resistance, wherein the nucleic acid is under control of the pathogen inducible synthetic promoter according to claim 1 , and subsequently regenerating a plant from the plant cell.
10. A vector or an expression cassette comprising a minimal promoter for producing a pathogen inducible synthetic promoter, wherein the minimal promoter consists essentially of a TATA-box, a transcription starting point, and a sequence motif dbrmwa, wherein the sequence motif dbrmwa is disposed downstream from the TATA-box and in front of the transcription starting point at which transcription of a nucleic acid to be regulated starts, and wherein the TATA-box is the binding location for the TATA-box binding protein (TBP) associated with transcription of said nucleic acid, and wherein the minimal promoter is operably linked to at least one cis regulatory element.
11. The vector or expression cassette according to claim 10 , wherein the sequence motif occurs two or more times in the minimal promoter.
12. The vector or expression cassette according to claim 10 , wherein the minimal promoter includes the nucleotide sequence according to one of SEQ ID NOS: 1-9 or derivatives thereof.
13. A pathogen inducible synthetic promoter, which is suitable for regulating the transcription of a nucleic acid, and includes at least one cis regulatory element and a minimal promoter,
wherein the minimal promoter is heterologous to the cis regulatory element,
wherein the minimal promoter consists essentially of a TATA-box, a transcription starting point, and a sequence motif dbrmwa,
wherein the sequence motif dbrmwa is disposed downstream from the TATA-box and in front of the transcription starting point at which transcription of the nucleic acid to be regulated starts, and
which includes, in addition to the minimal promoter, at least one cis-regulating element with the nucleotide sequence according to one of SEQ ID NOS: 10-15.
14. A plant cell, in which the expression cassette according to claim 10 has been integrated into DNA of the plant cell.
15. A transgenic plant comprising the plant cell according to claim 14 .
16. A seed of a transgenic plant comprising the expression cassette according to claim 10 .
17. A process for producing a pathogen resistant plant, comprising introducing in a plant cell a nucleic acid causing pathogen resistance, wherein the nucleic acid is the vector or the expression cassette according to claim 10 , and subsequently regenerating a plant from the plant cell.
18. A pathogen inducible synthetic promoter for regulating the transcription of a nucleic acid, which includes at least one cis regulatory element and a minimal promoter, wherein the minimal promoter is heterologous to the at least one cis regulatory element and wherein the minimal promoter comprises a sequence motif dbrmwa which is disposed downstream from a TATA-box and in front of a transcription starting point which are located on the minimal promoter and at which transcription of the nucleic acid to be regulated starts, and wherein the TATA-box is the binding location for the TATA-box binding protein (TBP) associated with transcription of said nucleic acid.
19. The vector or expression cassette according to claim 10 , wherein the minimal promoter is operably linked to a recombinant gene.
20. The vector or expression cassette according to claim 11 , wherein the minimal promoter is operably linked to a recombinant gene.
21. The vector or expression cassette according to claim 12 , wherein the minimal promoter is operably linked to a recombinant gene.