Biomimetic polymers and uses thereof
Biodegradable polymers incorporating biomolecules and methods of their use are provided. Certain aspects provide biomolecules crosslinked with diglycidyl esters. The disclosed compositions have numerous applications including cellular regeneration, wound healing, and cellular differentiation.
1. A polymer comprising repeating units — according to the following formula:
wherein R 1 is any alkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, or heterocycle wherein R 1 is optionally substituted with one or more functional group(s) selected from the group consisting of halide, alcohol, ether, thioether, tertiary amine, ketone, ester, and amide;
R 2 is any alkyl, cycloalkyl, aryl, or heteroaryl, wherein R 2 is optionally substituted with one or more functional group(s) selected from the group consisting of halide, alcohol, ether, thioether, tertiary amine, ketone, ester, and amide;
R 3 is a biomolecule selected from the group consisting of dopamine, glutamate, aspartate, histamine, norepinephrine, serotonin, taurine, glycine, 4-aminobutyrate, and methionine enkephalin, wherein R 3 is bound to R 2 via an amine bond; and
n is>1.
2. The polymer of claim 1 that is produced from diglycidyl ester which is produced from a dicarboxylic acid.
3. The polymer of claim 2 wherein the dicarboxylic acid is selected from the group consisting of adipic acid, aldaric acid, aspartic acid, azelaic acid, camphoric acid, dimercaptosuccinic acid, fumaric acid, glutamic acid, glutaric acid, isophthalic acid, itaconic acid, ketoglutaric acid, maleic acid, malic acid, malonic acid, mesoxalic acid, n-acetylglutamic acid, oxalic acid, oxaloacetic acid, phthalic acid, phthalic acids, pimelic acid, sebacic acid, suberic acid, succinic acid, tartaric acid, terephthalic acid, traumatic acid, dodecanedioic acid, chaetomellic acids, ceriporic acids, 1, 2-cyclohexane dicarboxylic acid, and 1,4-cyclohexane dicarboxylic acid.
4. The polymer of claim 1 , wherein the polymer is biodegradable.
5. The polymer of claim 1 , further comprising an amino spacer.
6. The polymer of claim 1 , wherein the polymer promotes axon growth relative to a control.
7. A polymer, produced by condensing monomers of dopamine with a diglycidyl ester, having the formula:
wherein R 1 and R 2 are independently C 1 -C 18 ; and
R 3 is selected from the group consisting of alkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, and heterocycle; and
wherein n is>1.
8. The polymer of claim 7 , wherein the diglycidyl ester is 1,2 cyclohexyldiglycidyl ester.
9. A method for inducing neurite growth comprising contacting neural cells with the polymer of claim 7 .
10. A method for inducing cell growth or regeneration comprising culturing one or more cells on the polymer of claim 1 .
11. A method for delivering nucleic acid to a cell comprising packaging the nucleic acid in the polymer of claim 1 and contacting the cell with the packaged nucleic acid.
12. A method for inducing cellular differentiation comprising:
culturing one or more cells on the polymer of claim 1 .
13. A method for delivering a nucleic acid to cell comprising combining the nucleic with the polymer of claim 1 and contacting a cell with the combination.
14. The polymer of claim 1 having the following structure
15. The polymer of claim 1 , wherein n>1,000.
16. The polymer of claim 1 , wherein n>10,000.
17. The polymer of claim 1 , wherein n>100,000.