Methods and compositions for pathogen detection using nanoparticle-fluorescent polymer complexes
Compositions, methods and related apparatus, as can be used for selective pathogen detection and identification.
1. A method of detecting the presence of a pathogen analyte, the method comprising:
providing a plurality of non-covalent complexes between a plurality of nanoparticles and a fluorescent polymer,
wherein each of the plurality of nanoparticles comprises an inner metallic core and a coating layer comprising a cationic ligand,
wherein the fluorescent polymer comprises an anionic group, and
wherein fluorescence of the polymer in the non-covalent complexes is quenched;
mixing a sample to be tested for the presence of the pathogen analyte with the plurality of non-covalent complexes under a condition such that, if the pathogen analyte is presence in the sample, at least some of the fluorescent polymer is displaced by the pathogen, thereby resulting in restoration of at least some of fluorescence of the fluorescent polymer; and
measuring a fluorescence pattern of the resulting sample to determine the presence of the pathogen analyte.
2. The method of claim 1 , wherein the plurality of non-covalent complexes comprise at least three non-covalent complexes between at least three different nanoparticles and a fluorescent polymer.
3. The method of claim 2 , wherein the cationic ligand comprises a quaternary ammonium ion.
4. The method of claim 1 , wherein the fluorescent polymer is a synthetic π-conjugated polymer.
5. The method of claim 4 , wherein the synthetic π-conjugated polymer comprises a structural unit of:
wherein R 1 and R 2 are independent selected from H, alkyl and oxa-substituted alkyl groups; R′ 1 , and R′ 2 are independently selected from H and alkyl groups; provided that at least one of R′ 1 and R′ 2 comprises a charged group.
6. The method of claim 5 , wherein at least one of R′ 1 , and R′ 2 comprises a carboxylate or a sulfate anion and a counter cation.
7. The method of claim 6 , wherein each of R′ 1 , and R′ 2 is:
8. The method of claim 5 , wherein at least one of R 1 , and R 2 comprises a poly(alkylene oxide) group.
9. The method of claim 8 , wherein each of R 1 , and R 2 is:
10. The method of claim 5 , wherein the coating layer of each of the nanoparticles has covalently bond thereon a cationic ligand having the structure of
wherein R is selected from:
11. The method of claim 1 , wherein measuring a fluorescence pattern of the resulting sample to determine the presence of the pathogen analyte comprises analyzing the fluorescent pattern by discriminate analysis.
12. A method of detecting the presence of a pathogen analyte, the method comprising:
providing a plurality of non-covalent complexes between a nanoparticle and a plurality of fluorescent polymers,
wherein the nanoparticle comprises an inner metallic core and a coating layer comprising a cationic ligand,
wherein each of the fluorescent polymers comprises an anionic group, and
wherein fluorescence of the fluorescent polymers in the non-covalent complexes is quenched;
mixing a sample to be tested for the presence of the pathogen analyte with the plurality of non-covalent complexes under a condition such that, if the pathogen analyte is present in the sample, at least some of the fluorescent polymers are displaced by the pathogen, thereby resulting in restoration of at least some of fluorescence of the fluorescent polymers; and
measuring a fluorescence pattern of the resulting sample to determine the presence of the pathogen analyte.
13. The method of claim 12 wherein, the plurality of non-covalent complexes comprise at least three non-covalent complexes between a nanoparticle and at least three different fluorescent polymers.
14. The method of claim 13 wherein the coating layer of the nanoparticle has covalently bond thereon a cationic ligand having the structure of
wherein R is selected from:
15. The method of claim 12 , wherein at least one of the fluorescent polymers is a synthetic π-conjugated polymer.
16. The method of claim 15 , wherein each of the π-conjugated polymers comprises a structural unit of:
wherein R 1 and R 2 are independently selected from H, alkyl and oxa-substituted alkyl groups; provided that at least one of R 1 and R 2 comprises a charged group.
17. The method of claim 16 , wherein at least one of R 1 , and R 2 comprises a carboxylate or a sulfate anion and a counter cation.
18. The method of claim 17 , wherein each of R 1 , and R 2 is:
19. The method of claim 12 , wherein at least one of the fluorescent polymers is a natural fluorescent protein.
20. The method of claim 19 , wherein the natural fluorescent polymer comprises a green fluorescent protein.
21. The method of claim 12 , wherein measuring a fluorescence pattern of the resulting sample comprises measuring a fluorescent pattern for each of the fluorescent polymers at the same wavelength.