Compositions for and methods of enhancing the immune response to antigens
Compositions comprising the compound of formula are provided herein. Also provided are methods of enhancing an immune response of a subject to an antigen by administering the antigen and the composition. The enhanced immune response may be an humoral immune response, a CD4+ T cell response, a CD8+ T cell response or result in activation of antigen presenting cells. Methods of enhancing the immune response by intramuscular administration of an antigen and the composition including the compound of formula I are also provided.
1. A composition comprising an antigen and a compound of formula I:
wherein R 1 is H or —OH, R 2 is —H or —OH, x is an integer from 18 to 26, and n is an integer from 10 to 15.
2. The composition of claim 1 , wherein x is 23.
3. The composition of claim 2 , wherein n is 13.
4. The composition of claim 1 , wherein x is 21.
5. The composition of claim 4 , wherein n is 13.
6. A pharmaceutical composition comprising the composition of claim 1 .
7. A method of enhancing an immune response of a subject to an antigen, comprising administering a composition comprising an antigen and a compound of formula I.
wherein R 1 is H or —OH, R 2 is —H or —OH, x is an integer from 18 to 26, and n is an integer from 10 to 15 to the subject, wherein the immune response of the subject to the antigen is enhanced relative to an immune response of a control to the antigen.
8. The method of claim 7 , wherein the immune response of the subject is enhanced at least 50% relative to the control.
9. The method of claim 7 , wherein the immune response of the subject is enhanced at least 100% relative to the control.
10. Then method of claim 7 , wherein the immune response of the subject is enhanced at least 1000% relative to the control.
11. The method of claim 7 , wherein the composition is administered via a route selected from the group consisting of intravenously, intramuscularly, subcutaneously, intradermally, intraperitoneally, intranasally and by inhalation.
12. The method of claim 7 , wherein the immune response is a humoral immune response.
13. The method of claim 12 , wherein the humoral immune response comprises production of IgG antibodies.
14. The method of claim 12 , wherein the humoral immune response comprises production of IgA antibodies.
15. The method of claim 7 , wherein the immune response comprises a CD4+ T cell response.
16. The method of claim 15 , wherein the enhanced CD4+ T cell response comprises activation of CD4+ T lymphocytes.
17. The method of claim 16 , wherein activation of the CD4+ T lymphocytes comprises an increase in a Th1 immune response.
18. The method of claim 16 , wherein activation of the CD4+ T lymphocytes comprises an increase in a Th2 immune response.
19. The method of claim 16 , wherein activation of the CD4+ T lymphocytes comprises an increase in both a Th1 and a Th2 immune response.
20. The method of claim 7 , wherein the immune response comprises a CD8+ T cell response.
21. The method of claim 20 , wherein the enhanced CD8+ T cell response comprises activation of the CD8+ T lymphocytes.
22. The method of claim 21 , wherein the activation of the CD8+ T lymphocytes comprises an increase in cytotoxic response.
23. A method of enhancing activation of antigen presenting cells of a subject to an antigen, comprising administering a composition comprising the antigen and a compound of formula I:
wherein R 1 is H or —OH, R 2 is —H or —OH, x is an integer from 18 to 26, and n is an integer from 10 to 15 to the subject, wherein the immune response of the subject to the antigen is enhanced relative to activation of antigen presenting cells of a control to the antigen.
24. The method of claim 7 , comprising administering the composition to the subject intramuscularly.
25. The method of claim 24 , wherein the immune response of the subject is enhanced at least 50% relative to the control.
26. The method of claim 24 , wherein the immune response of the subject is enhanced at least 100% relative to the control.
27. The method of claim 24 , wherein the immune response of the subject is enhanced at least 1000% relative to the control.
28. The method of claim 7 , wherein n is 13 and x is 23.
29. The method of claim 7 , wherein n is 13 and x is 21.
30. The method of claim 23 , wherein n is 13 and x is 23.
31. The method of claim 23 , wherein n is 13 and x is 21.