Activity of recombinant equine follicle stimulating hormone
The present invention provides methods of producing biologically active recombinant eFSH analogs and methods of increasing reproduction in mammals, particularly equine, using recombinant eFSH analogs. Also provided are methods of producing biologically active single chain recombinant eFSH. The recombinant eFSH analogs of the present invention increase superovulation, follicular development, embryo development, and reproductive efficiency in horses, cattle and other ungulates.
1. A method of stimulating follicular development or superovulation in an ungulate comprising administering an effective amount of an equine follicle stimulating hormone (eFSH) analog to said ungulate, wherein said eFSH analog comprises a first polypeptide having at least 95% homology with SEQ ID NO 5, and a second polypeptide having at least 95% homology with SEQ ID NO 6, wherein said first and second polypeptides are covalently linked by an equine chorionic gonadotropin carboxy terminal peptide (CTP) having at least 90% homology with SEQ ID NO 7, wherein said effective amount is between approximately 200 μg and approximately 1.0 mg administered daily to said ungulate for approximately 4 to 9 days between day 6 to day 16 after ovulation.
2. The method of claim 1 wherein said ungulates are equine.
3. The method of claim 1 further comprising inseminating said ungulate between 2 to 8 days after administrating said eFSH analog.
4. The method of claim 1 wherein said eFSH analog has at least 95% homology with the full length sequence of SEQ ID NO 8.
5. The method of claim 1 wherein said effective amount is between approximately 500 μg and approximately 850 μg.
6. The method of claim 1 wherein a dose of the eFSH analog is administered daily to said ungulate for approximately 5 to 8 days in a single estrus cycle.
7. The method of claim 6 wherein a dose of the eFSH analog is administered twice daily to said ungulate for approximately 5 to 8 days in a single estrus cycle.