Polypeptides having endoglucanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. An isolated polypeptide having endoglucanase activity, selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2;
(b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii); and
(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.
2. The polypeptide of claim 1 , comprising or consisting of the amino acid sequence of SEQ ID NO: 2; or a fragment thereof having endoglucanase activity.
3. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising or consisting of the nucleotide sequence of SEQ ID NO: 1; or a subsequence thereof encoding a fragment having endoglucanase activity.
4. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pTter16A which is contained in E. coli NRRL B-50081.
5. The polypeptide of claim 1 , comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.
6. The polypeptide of claim 5 , comprising an amino acid sequence having at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.
7. The polypeptide of claim 2 , comprising or consisting of the amino acid sequence of SEQ ID NO: 2.
8. The polypeptide of claim 1 , comprising or consisting of the mature polypeptide of SEQ ID NO: 2.
9. The polypeptide of claim 1 , which is encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii).
10. The polypeptide of claim 9 , which is encoded by a polynucleotide that hybridizes under at least very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii).
11. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.
12. The polypeptide of claim 11 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 97% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.
13. The polypeptide of claim 3 , which is encoded by a polynucleotide comprising or consisting of the nucleotide sequence of SEQ ID NO: 1.
14. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising or consisting of the mature polypeptide coding sequence of SEQ ID NO: 1.