Breed-specific haplotypes for polled phenotypes in cattle
View Patent ↗The presence of horns within commercial cattle populations increases the chances of injuries. The Polled (hornless) condition in cattle has existed since domestication, and it has been selected by breeders because of its economic importance and ease of management. A dominant mutation is believed to cause the polled phenotype. The present invention relates to the identification of breed-specific single nucleotide polymorphisms (SNPs) and their haplotypes, in the bovine genome, in or near gene(s) encoding polypeptides associated with the Polled phenotyped in beef production. The invention further encompasses methods and systems, including network-based processes, to manage the SNP data, haplotype data and other data relating to specific animals and herds of animals, veterinarian care, diagnostic and quality control data and management of livestock which, based on genotyping, have predictable productions quality traits, husbandry conditions, animal welfare, food safety information, audit of existing processes and data from field location.
1. A method for identifying a bovine animal having Polled phenotype, as compared to a general population of bovine animals, comprising:
(a) obtaining a biological sample from said bovine animal;
(b) subjecting the sample to nucleotide sequence analysis to obtain sufficient nucleotide sequence information to provide the identity of the nucleotides at positions corresponding to the following SEQ ID NO: 43 positions: 1356048, 1872077, 1872200, 2101528, 2214142, 2220054, 2220178, 2225310, 2288436, 2332892, 2333140, 2338338, 2816109, 2816370, 1013610, 1352705, 1352905, 1431709, 1438531, 1731905, 2128551, 2640799, 2648129, 2650867, 2651006, 2657651, 2676311, and 2820224;
(c) detecting in said animal a haplotype of the alleles present at at least three nucleotides or a genotype of the alleles present at at least five nucleotides, wherein the at least three or at least five nucleotides in the haplotype or the genotype are nucleotides detected at the positions analyzed in part (b), wherein said haplotype or said genotype is sufficient to conclude that the animal will exhibit the Polled phenotype;
(d) identifying the animal as having the Polled phenotype based on the presence the haplotype comprised of at least three of said positions or the genotype comprised of at least five positions.
2. The method of claim 1 wherein:
(a) the animal is of the Limousin breed and a three SNP haplotype is detected;
(b) the animal is of the Hereford breed and a five SNP haplotype is detected;
(c) the animal is of the Shorthorn breed and an eight SNP haplotype is detected;
(d) the animal is of the Charolais breed and a six SNP haplotype is detected;
(e) the animal is of the Jersey breed and a four SNP haplotype is detected; or
(f) the animal is of the Gelbvieh breed and an eight SNP haplotype is detected.
3. The method of claim 2 , wherein the three SNP haplotype consists of alleles present at nucleotide positions 1872077, 2288436, and 2332892.
4. The method of claim 2 , wherein the five SNP haplotype consists of alleles present at nucleotide positions 1356048, 2101528, 2214142, 2333140, and 2816109.
5. The method of claim 2 , wherein the eight SNP haplotype for the Galbvieh breed consists of alleles present at nucleotide positions 1356048, 2220054, 2288436, 2332892, 2333240, 2816109, 2128551, and 2650867.
6. The method of claim 2 , wherein the six SNP haplotype consists of alleles present at nucleotide positions 1872077, 2214142, 2332892, 2816109, 1013610, and 1731905.
7. The method of claim 2 , wherein the eight SNP haplotype for the Shorthorn breed consists of alleles present at nucleotide positions 2101528, 2128551, 2214142, 2288436, 2332892, 2333140, 2816109 and 1731905.
8. The method of claim 2 , wherein the four SNP haplotype consists of alleles present at nucleotide positions 1256048, 2101528, 2288436, and 233140.
9. A method of sub-grouping bovine animals comprising
(a) identifying bovine animals having the Polled phenotype using the method set forth in claim 1 or claim 2 , and
(b) sub-grouping the animals according to the haplotype or genotype that was detected.