Method and apparatus for chromatography using spherical silica gel

1. A method of performing liquid chromatography on a sample, comprising the steps of:

receiving a target time of run resolution input, the target time of run resolution based on a target resolution in a target time;

determining if a chromatographic column containing shape stabilized packing is present;

applying sample to the chromatographic column containing shape stabilized packing;

pumping liquid phase through the chromatographic column containing shape stabilized packing at a rate of flow at least 20% to 50% greater than a standard rate of flow to obtain the target time of run resolution.

2. A method of performing liquid chromatography in accordance with claim 1 wherein the liquid chromatography is flash chromatography.

3. A method in accordance with claim 2 wherein a liquid chromatographic column has an inner diameter greater than 20 mm.

4. A method in accordance with claim 1 wherein at least one of a rate of flow and a gradient slope is selected in accordance with the resolution required.

5. A cost effective liquid chromatographic method, comprising the steps of:

receiving a target time of run resolution input, the target time of run resolution based on a target resolution in a target time;

running at least one pilot run on a sample, the pilot run including a run time, a gradient slope, and a flow rate;

determining if the at least one pilot run met the target time of run resolution;

shortening a run time, increasing a gradient slope and increasing a flow rate for samples for which the at least one pilot run provides a time of run resolution greater than the target time of run resolution.

6. A method of liquid chromatography, comprising the steps of:

programming at least one gradient run with at least one gradient profile for a sample;

determining whether the at least one gradient profile provides a target resolution and a target time of run resolution for the sample;

performing chromatography on the sample with the at least one gradient run for samples in which the target resolution and target time of run resolution were met with the at least one gradient run and increasing a rate of flow for samples in which the target resolution was exceeded whereby the time of run resolution is decreased.

7. The method of claim 6 in which the step of programming at least one gradient run includes the step of using TLC to determine whether the target resolution and target time of run resolution are met.

8. The method of claim 6 in which the step of programming at least one gradient run includes the step of making a pilot run to determine whether the target resolution and the target time of run resolution are met.

9. The method of claim 6 in which the step of programming at least one gradient run includes the steps of:

increasing a rate of flow of solvent by at least 10 percent and a gradient slope by at least 10 percent for a first trial run;

decreasing the rate of flow of solvent and decreasing the gradient slope for pilot runs in which the target resolution is not met and increasing the rate of flow and the gradient slope for pilot runs in which the target resolution is exceeded.

10. The method of liquid chromatography of claim 6 wherein determining whether the at least one gradient profile provides a target resolution and a target time of run resolution for the sample further comprises the steps of:

measuring a first separation between a first peak and a second peak;

receiving the target time of run resolution;

comparing the first separation with the target time of run resolution;

increasing a flow rate of a solvent to achieve the target time of run resolution if the target resolution has not been met by the first separation;

measuring a second separation between the first peak and the second peak; and

increasing a gradient to achieve the target time of run resolution if the target resolution has not been met by the second separation.

11. The method of liquid chromatography of claim 6 , wherein determining whether the at least one gradient profile provides a target resolution and a target time of run resolution for the sample further comprises the steps of:

programming at least one gradient run with at least one gradient profile, the at least one gradient profile including the target time of run resolution; and

altering the at least one gradient profile by increasing a flow rate of a solvent to improve the separation between a target component and a closest impurity.

12. A method of identifying, separating or purifying a target component using column chromatography, comprising the steps of:

determining resolution with a standard gradient;

identifying a poorly resolved target compound with a high equivalent plate height;

making a chromatographic run with a gradient no greater than the standard gradient but with a flow rate greater than a standard flow rate; and

separating or purifying a well resolved target component, making a chromatographic run at a higher flow rate and a gradient no steeper than the standard gradient.