Capillary electrophoresis device
View Patent ↗An object of the present invention is to prevent a variation in heat dissipating effect of a capillary between a holder part and an oven, to improve reproducibility of migration time, and to reduce a variation of migration time among capillaries in a single electrophoresis run. A cylindrical wall is formed in an upper part of a septa that covers a container holding a solution, and the cylindrical wall surrounds a capillary hole through which a capillary penetrates. Accordingly, the capillary is prevented from being directly affected by wind generated between the septa and the oven.
1. A capillary electrophoresis device, comprising:
a capillary;
a power supply that applies a voltage to both ends of the capillary;
an oven that houses a main part of the capillary and maintains an ambient temperature of the main part constant;
a detector that irradiates with an exciting light a sample separated by electrophoresis inside of the capillary and detects fluorescence from the sample;
a load header that is fixed to the oven and has a hollow capillary electrode mounted thereto, a tip part of the capillary being inserted into the capillary electrode;
a holder part in which any one of a sample and an electrolytic solution is held; and
an auto sampler that transfers the holder part, wherein:
the holder part comprises: a container that houses the one of the sample and the electrolytic solution, and has an opening in an upper part thereof; and a septa having a capillary hole through which the capillary electrode projecting from the load header penetrates, the septa covering the upper part opening of the container,
the septa has in an upper part thereof a structure that surrounds the capillary electrode so that air circulating inside of the device does not directly blow onto the capillary electrode, and
the structure that surrounds the capillary electrode is a cylindrical wall that is installed so as to surround a plurality of the capillary holes.
2. The capillary electrophoresis device according to claim 1 , wherein the septa is made of a resin material having high electric insulation properties.