Recombinant infectious laryngotracheitis virus and uses thereof
The present invention provides a recombinant, attenuated infectious laryngotracheitis virus comprising the infectious laryngotracheitis viral genome which contains a deletion in the glycoprotein gG gene. This attenuated virus is useful as a vaccine against infectious laryngotracheitis virus. The present invention also provides a recombinant, attenuated infectious laryngotracheitis virus comprising the infectious laryngotracheitis viral genome which contains a deletion in the US2 gene, UL47-like gene, ORF4 gene or glycoprotein g60 gene. The present invention also provides a method for distinguishing chickens or other poultry vaccinated with a recombinant infectious laryngotracheitis virus which produces no glycoprotein gG from those infected with a naturally-occurring infectious laryngotracheitis virus.
1. A recombinant infectious laryngotracheitis virus comprising the infectious laryngotracheitis viral genome which contains a deletion in the unique short region of the viral genome, wherein the deletion is in the glycoprotein g60 gene.
2. The recombinant infectious laryngotracheitis virus of claim 1 , wherein a foreign gene is inserted in the glycoprotein g60 gene.
3. A recombinant infectious laryngotracheitis virus comprising the infectious laryngotracheitis viral genome which contains a deletion in the unique short region of the viral genome, wherein the deletion is in a gene selected from a group consisting of the US2 gene, the UL47-like gene, and the glycoprotein g60 gene.
4. A recombinant infectious laryngotracheitis virus of claim 3 , wherein the foreign gene is inserted in the gene selected from a group consisting of the US2 gene, UL-47 like gene, ORF4 gene and glycoprotein g60 gene.
5. The recombinant infectious laryngotracheitis virus of claim 4 , wherein the foreign gene encodes an antigenic polypeptide.
6. The recombinant infectious laryngotracheitis virus of claim 5 , wherein the antigenic polypeptide, when introduced into the host cell, induces production of protective antibodies against an avian disease causing agent from which the antigen is derived or derivable.
7. The recombinant infectious laryngotracheitis virus of claim 6 , wherein the antigenic polypeptide is derived from or derivable from a group consisting of infectious bronchitis virus, Newcastle disease virus, infectious bursal disease virus, and Marek's disease virus.
8. The recombinant infectious laryngotracheitis virus of claim 4 , wherein the foreign gene is under control of a heterologous upstream promoter.
9. The recombinant infectious laryngotracheitis virus of claim 8 , wherein the promoter is selected from a group consisting of HCMV IE promoter, PRV gX promoter, and BHV-1.1 VP8 promoter.