Method of obtaining an active ingredient increasing cutaneous cell and tissue longevity, active ingredients and compositions
View Patent ↗Method for obtaining of an active principle from Myrtus communis in order to increase the longevity of the cells and tissues of the skin.
1. A method of increasing cutaneous tissue longevity and cutaneous cellular longevity, comprising applying a Myrtus communis active principle having:
a percentage of dry materials ranging between 20 and 200 g/L,
a pH ranging between 3.0 and 6.0,
a content of total sugars ranging between 5 and 64 g/L,
and a content of total phenols ranging between 0.5 and 10.5 g/L
to human skin, wherein the active principle is obtained by a method comprising:
(a) solubilizing Myrtus communis leaf powder in water in acid medium to form a solution,
(b) carrying out simultaneous and/or successive enzymatic hydrolysis/hydrolyses on the solution of (a),
(c) separating the soluble and insoluble phases of the hydrolyzed solution of (b) by filtration, centrifugation or decantation, to obtain an enzyme-containing soluble phase,
(d) inactivating the soluble phase of (c) by thermal treatment,
(e) purifying by clarification, thereby providing an active fraction,
(f) concentrating the active fraction to provide a concentrated solution of the active fraction, and
(g) filtering the concentrated solution.
2. The method according to claim 1 , wherein the percentage of dry materials ranges between 28 and 42 g/l.
3. The method according to claim 1 , wherein the pH ranges between 3.5 and 4.5.
4. The method according to claim 1 , wherein the content of total sugars ranges between 7 and 14 g/l.
5. The method according to claim 1 , wherein the content of total phenols ranges between 0.9 and 2.3 g/l.
6. The method according to claim 1 , wherein the sugar content comprises glucose and/or galacturonic acid.
7. The method according to claim 1 , wherein the phenol content comprises hydroxybenzoics and/or flavonols.
8. The method according to claim 1 , wherein the sugar content comprises glucose and/or galacturonic acid oligosaccharides.