IP Library Granted Patent US 8,137,707
Granted Patent B2
US 8,137,707 · App. 12/373,215 · Granted Mar 20, 2012

Method of obtaining an active ingredient increasing cutaneous cell and tissue longevity, active ingredients and compositions

Assignee: Societe Industrielle Limousine d'Application Biologique, Dite Silab
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Quick Facts
Patent No.
US 8,137,707
App. No.
12/373,215
Granted
Mar 20, 2012
Kind
B2
Abstract

Method for obtaining of an active principle from Myrtus communis in order to increase the longevity of the cells and tissues of the skin.

Claims (20)

1. A method of increasing cutaneous tissue longevity and cutaneous cellular longevity, comprising applying a Myrtus communis active principle having:

a percentage of dry materials ranging between 20 and 200 g/L,

a pH ranging between 3.0 and 6.0,

a content of total sugars ranging between 5 and 64 g/L,

and a content of total phenols ranging between 0.5 and 10.5 g/L

to human skin, wherein the active principle is obtained by a method comprising:

(a) solubilizing Myrtus communis leaf powder in water in acid medium to form a solution,

(b) carrying out simultaneous and/or successive enzymatic hydrolysis/hydrolyses on the solution of (a),

(c) separating the soluble and insoluble phases of the hydrolyzed solution of (b) by filtration, centrifugation or decantation, to obtain an enzyme-containing soluble phase,

(d) inactivating the soluble phase of (c) by thermal treatment,

(e) purifying by clarification, thereby providing an active fraction,

(f) concentrating the active fraction to provide a concentrated solution of the active fraction, and

(g) filtering the concentrated solution.

2. The method according to claim 1 , wherein the percentage of dry materials ranges between 28 and 42 g/l.

3. The method according to claim 1 , wherein the pH ranges between 3.5 and 4.5.

4. The method according to claim 1 , wherein the content of total sugars ranges between 7 and 14 g/l.

5. The method according to claim 1 , wherein the content of total phenols ranges between 0.9 and 2.3 g/l.

6. The method according to claim 1 , wherein the sugar content comprises glucose and/or galacturonic acid.

7. The method according to claim 1 , wherein the phenol content comprises hydroxybenzoics and/or flavonols.

8. The method according to claim 1 , wherein the sugar content comprises glucose and/or galacturonic acid oligosaccharides.

Assignments (2)
CHANGE OF ADDRESS Recorded Jan 28, 2025
From: SOCIETE INDUSTRIELLE LIMOUSINE D'APPLICATION BIOLOGIQUE
To: SOCIETE INDUSTRIELLE LIMOUSINE D'APPLICATION BIOLOGIQUE
Reel/Frame 070028/0334 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 19, 2009
From: PAUFIQUE, JEAN
To: SOCIETE INDUSTRIELLE LIMOUSINE D'APPLICATION BIOLOGIQUE, DITE SILAB
Reel/Frame 022704/0575 →
Priority Claims (1)
FR 06 52900 · Jul 11, 2006 · national
Continuity (1)
Related Publication 20100009018A1 · Jan 14, 2010