Thermophilic and thermoacidophilic metabolism genes and enzymes from
Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering metabolism in a cell using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.
1. An expression vector comprising an isolated polynucleotide encoding a polypeptide having at least 90% sequence identity to SEQ ID NO:1395.
2. The expression vector of claim 1 , wherein the expression vector comprises a polynucleotide having at least 90% identity to SEQ ID NO:1396.
3. The expression vector of claim 1 , wherein the encoded polypeptide has L-lactate dehydrogenase enzymatic activity.
4. An expression vector comprising a polynucleotide encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:1395.
5. The expression vector of claim 4 , wherein the encoded polypeptide has L-lactate dehydrogenase enzymatic activity.
6. A method of modulating or altering metabolism in a cell, the method comprising: providing the expression vector of claim 3 to the cell and expressing the encoded polypeptide in the cell.
7. The method according to claim 6 , further comprising glycosylating, or otherwise post-translationally modifying the encoded peptide in the cell.