Concentrated aqueous silk fibroin solution and use thereof
The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.
1. A composition comprising a silk fibroin protein solution and a therapeutic agent wherein said silk fibroin protein solution consists essentially of at least 15% wt silk fibroin protein and water.
2. The composition of claim 1 , wherein said silk fibroin protein is obtained from a solution of dissolved silkworm silk, genetically engineered silk, or dissolved spider silk.
3. The composition of claim 1 , wherein said silk fibroin protein solution consists essentially of at least 20% wt silk fibroin and water.
4. The composition of claim 1 , wherein said silk fibroin protein solution consists essentially of at least 25% wt silk fibroin and water.
5. The composition of claim 1 , wherein said silk fibroin protein solution consists essentially of at least 30% wt silk fibroin and water.
6. The composition of claim 1 , wherein the therapeutic agent is a small molecule, a protein, a peptide, a polysaccharide, a glycoprotein, a lipoprotein, or a nucleic acid.
7. The composition of claim 1 , wherein the therapeutic agent is a small molecule selected from the group consisting of vitamins, sedatives, hypnotics, antibiotics, antiviral agents, steroids, growth factor inhibitors, growth factor promoters, prostaglandins and radiopharmaceuticals.
8. The composition of claim 1 , wherein the therapeutic agent is selected from the group consisting of anti-infectives, chemotherapeutic agents, anti-rejection agents, analgesics and analgesic combinations, anti-inflammatory agents, hormones, anti-angiogenic agents, anti-thrombotics, anti-metabolics, antimitotics, fibrinolytics, and growth factors.
9. The composition of claim 8 , wherein the growth factor is selected from the group consisting of bone morphogenic proteins, bone morphogenic-like proteins, epidermal growth factor, fibroblast growth factor, platelet derived growth factor, insulin-like growth factor, transforming growth factors, and vascular endothelial growth factor.
10. The composition of claim 1 , wherein the therapeutic agent is an antibody.
11. The composition of claim 1 , wherein the therapeutic agent is present as a liquid or a finely divided solid.
12. The composition of claim 1 , wherein the therapeutic agent further comprises a pharmaceutically acceptable carrier.