Bacillus strains useful for animal odor control
View Patent ↗A composition is provided that includes Bacillus subtilis 2084 (NRRL B-50013) or a strain having all of the identifying characteristics of the Bacillus subtilis 2084 (NRRL B-50013), B. subtilis 27 (NRRL B-50105) or a strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105), and B. licheniformis 21 (NRRL B-50134) or a strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134). Animal bedding that includes the Bacillus strains is also provided, as well as a method of making the animal bedding. Also provided are methods of controlling odors from animal waste. A method of making a composition including the Bacillus strains is also provided.
1. A composition comprising:
an isolated Bacillus subtilis 2084 (NRRL B-50013) or an isolated strain having all of the identifying characteristics of the Bacillus subtilis 2084 (NRRL B-50013);
an isolated B. subtilis 27 (NRRL B-50105) or an isolated strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105); and
an isolated B. licheniformis 21 (NRRL B-50134) or an isolated strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134).
2. The composition of claim 1 , wherein the amount of each strain is about the same.
3. The composition of claim 1 , further comprising at least one carrier capable of binding ammonia.
4. The composition of claim 3 , wherein the carrier is selected from the group consisting of comprise limestone, clay, zeolite, diatomaceous earth, clintpolite, bentonite, and Fuller's earth.
5. A method of controlling odors and ammonia from animal waste, the method comprising applying the composition of claim 3 to an area where an animal is housed, wherein application of the composition controlling odors and ammonia in the animal waste.
6. The composition of claim 1 , further comprising at least one water-soluble carrier.
7. The composition of claim 6 , wherein the carrier is selected from the group consisting of Baker's sugar, maltodextrin M100, and baylith.
8. The composition of claim 1 , further comprising bentonite, Fuller's earth, mineral oil, and baylith.
9. The composition of claim 1 , further comprising an absorbent material.
10. A composition comprising:
an isolated Bacillus subtilis 2084 (NRRL B-50013) or an isolated strain having all of the identifying characteristics of the Bacillus subtilis 2084 (NRRL B-50013);
an isolated B. subtilis 27 (NRRL B-50105) or an isolated strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105);
an isolated B. licheniformis 21 (NRRL B-50134) or an isolated strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134); and
an absorbent material, wherein the absorbent material is cat litter.
11. An animal bedding comprising:
an isolated Bacillus subtilis 2084 (NRRL B-50013) or an isolated strain having all of the identifying characteristics of the Bacillus subtilis 2084 (NRRL B-50013);
an isolated B. subtilis 27 (NRRL B-50105) or an isolated strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105);
an isolated B. licheniformis 21 (NRRL B-50134) or an isolated strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134); and
animal bedding.
12. The animal bedding of claim 11 , wherein the animal bedding is selected from the group consisting of wood shavings, corn cob bedding, paper bedding, straw, and wood pellets.
13. The animal bedding of claim 11 , further comprising at least one water-soluble carrier.
14. A method of controlling odors and ammonia from animal waste, the method comprising applying the animal bedding of claim 11 to an area where an animal is housed, wherein application of the composition controlling odors and ammonia in the animal waste.
15. A method of making animal bedding, the method comprising applying Bacillus subtilis 2084 (NRRL B-50013) or a strain having all of the identifying characteristics of the Bacillus subtilis 2084 (NRRL B-50013), B. subtilis 27 (NRRL B-50105) or a strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105), and B. licheniformis 21 (NRRL B-50134) or a strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134) to animal bedding.
16. The method of claim 15 , further comprising:
adding a water-soluble carrier to the strains; and
rehydrating the strains, wherein the rehydrated strains are applied to the animal bedding.
17. The method of claim 15 , wherein the strains are applied to provide substantially the same cfu of each strain.
18. The method of claim 15 , further comprising packaging the animal bedding, wherein the strains are applied prior to packaging the animal bedding.
19. The method of claim 15 , wherein the animal bedding is selected from the group consisting of wood shavings, corn cob bedding, paper bedding, straw, and wood pellets.
20. A method of making a composition, the method comprising:
combining Bacillus subtilis 2084 (NRRL B-50013) or a strain having all of the identifying characteristics of the Bacillus subtilis 2084 (NRRL B-50013) with B. subtilis 27 (NRRL B-50105) or a strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105), and with B. licheniformis 21 (NRRL B-50134) or a strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134)
to form the three-strain composition.
21. The method of claim 20 , further comprising freeze drying each strain.
22. The method of claim 21 , further comprising adding the freeze-dried strain to at least one water-soluble carrier.
23. The method of claim 21 , further comprising adding the freeze-dried strain to at least one carrier that is capable of binding ammonia.